Microwave-assisted preparation of ZnFeO-Ag/rGO nanocomposites for amplification signal detection of alpha-fetoprotein.

In this study, a novel signal-amplified immunosensor was designed by using a microwave-assisted self-assembly method to synthesize ZnFeO-Ag/rGO nanocomposites. The conductivity of ZnFeO-rGO nanocomposites was significantly improved due to the effective inhibition of rGO accumulation by the insertion of ZnFeO and Ag nanoparticles (NPs) into graphene sheets. Excellent sensitivity and reproducibility were achieved through the microwave-assisted preparation of ZnFeO-Ag/rGO nanocomposites as a substrate, with the Ag NPs enhancing the signal because of the effective conductive matrix. The layer assembly process of the immunosensor was verified by cyclic voltammetry and electrochemical impedance spectroscopy. Under optimal conditions, the fabricated immunosensor showed good linearity over a wide concentration range from 1 pg mL to 200 ng mL with a low detection limit of 0.98 pg mL, and exhibited excellent specificity, good stability, and reproducibility. These qualities can contribute to the successful application of a label-free immunosensor in the detection of AFP in human serum.