Hispidulin, a natural compound present in herbs, has anti-cancer effects. Here, we investigated whether hispidulin sensitizes human carcinoma cells to apoptosis induced by TRAIL. Sub-lethal dosages of TRAIL alone and hispidulin alone does not increase apoptosis, but hispidulin increases sensitivity to TRAIL, resulting in induction of apoptosis in hispidulin plus TRAIL-treated cancer cells. In addition, combined treatment with hispidulin and TRAIL also reduced tumor growth and increased apoptosis in xenograft models. However, hispidulin did not alter cell viability in human renal normal mesangial cells and human skin fibroblast. Hispidulin markedly increased the BH3-only proteins Bim at the post-translational levels. Depletion of Bim with siRNA significantly blocked hispidulin plus TRAIL-induced apoptosis. Furthermore, we found that activation of AMPK by hispidulin has a crucial role in Bim proteins stability through up-regulation of USP51 expression. Our findings suggest that USP51-dependent stabilization of Bim by AMPK activation plays a critical role in hispidulin-mediated sensitization of cancer cells to apoptosis induced by TRAIL.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6966507 | PMC |
| http://dx.doi.org/10.3390/cancers11121960 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
LC-MS/MS-based metabolic profiling: unraveling the impact of varying degrees of curing on metabolite transformations in tobacco.
Front Plant Sci
November 2024
College of Life Science, Guizhou Normal University, Guiyang, China.
The curing process regulates metabolite transformations of leaves and significantly influences the formation of tobacco quality. This study investigated the major physicochemical compositions and metabolic profiles under normal curing (NC), excessive curing (EC), and insufficient curing (IC) treatments. The results indicated that the contents of nicotine, nitrogen, potassium, and chlorine remained stable among treatments, while the sugar content in EC was significantly lower than in IC.
View Article and Find Full Text PDFThe Role of in Regulating Internal Browning Involves Flavonoid Biosynthesis and the Endophytic Fungal Community of Pineapple.
J Fungi (Basel)
November 2024
Guangdong Province Key Laboratory of Postharvest Physiology and Technology of Fruit and Vegetables, College of Horticulture, South China Agricultural University, Guangzhou 510642, China.
Endophytic fungi are commonly used to control plant diseases, overcoming the drawbacks of chemical agents. The internal browning (IB) of postharvest pineapple fruit, a physiological disease, leads to quality losses and limits industrial development. This work investigated the relationship among the effects of (An) on IB controlling, flavonoid metabolism and the endophytic fungal community of pineapple through metabolomics, transcriptomics, microbiomics and microorganism mutagenesis technology.
View Article and Find Full Text PDFEthyl acetate extract of Artemisia argyi improves the resistance of cotton to Verticillium dahliae by activating the immune response.
Plant Physiol Biochem
December 2024
School of Pharmacy, Hubei University of Chinese Medicine, 430065, Wuhan, China; Hubei Shizhen Laboratory, Hubei University of Chinese Medicine, 430065, Wuhan, China. Electronic address:
Verticillium wilt, a significant pathogen affecting cotton, has historically been challenging to control, posing a substantial threat to the sustainable development of the cotton industry. This study demonstrates that resistance to Verticillium dahliae in cotton can be enhanced by treating the roots with an ethyl acetate extract (EAAA) extracted from Artemisia argyi. The mechanisms by which EAAA activates immunity in cotton were elucidated by examining the expression levels of resistance genes post-treatment, evaluating salicylic acid (SA) and jasmonic acid (JA) levels, analyzing transcriptome data, and employing virus-induced gene silencing (VIGS) technology.
View Article and Find Full Text PDFComputer-Aided Discovery of Compounds With Anti-Schistosomal Potential.
Biomed Eng Comput Biol
November 2024
Department of Biology, School of Natural Sciences and Mathematics, Chinhoyi University of Technology, Chinhoyi, Zimbabwe.
Schistosomiasis, which causes over 200 000 deaths annually, has since the 1970s been controlled by praziquintel. The reliance on a single drug to combat schistosomiasis, and reports of laboratory resistance to the drug, has created an urgent need in the scientific community to develop new chemotherapies to complement or supplement praziquantel. Medicinal plants are a potential reservoir of compounds with schistosomicidal activity.
View Article and Find Full Text PDFScreening Anti-Rheumatoid Arthritis Synovitis Effective Ingredients of Total Flavonoid From Artemisia argyi Folium Based on Spectrum-Effect Relationship.
Phytochem Anal
November 2024
Department of Pharmacy, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
Introduction: Flavonoids are the main nonvolatile component responsible for the anti-rheumatoid arthritis (RA) synovitis activities of Artemisia argyi Folium. However, the effective ingredient remains unidentified. Spectrum-effect relationships analysis was a reliable and efficient strategy for herbal effective ingredient discovery.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!