Bioinformatics Analysis of the Lipoxygenase Gene Family in Radish () and Functional Characterization in Response to Abiotic and Biotic Stresses.

Lipoxygenases (LOXs) are non-heme iron-containing dioxygenases involved in many developmental and stress-responsive processes in plants. However, little is known about the radish LOX gene family members and their functions in response to biotic and abiotic stresses. In this study, we completed a genome-wide analysis and expression profiling of genes under abiotic and biotic stress conditions. We identified 11 genes, which encoded conserved domains, and classified them in 9-LOX and 13-LOX categories according to their phylogenetic relationships. The characteristic structural features of 9-LOX and 13-LOX genes and the encoded protein domains as well as their evolution are presented herein. A qRT-PCR analysis of expression levels in the roots under simulated drought, salinity, heat, and cold stresses, as well as in response to a infection, revealed three tandem-clustered genes that are involved in responses to various environmental stresses via the jasmonic acid pathway. Our findings provide insights into the evolution and potential biological roles of RsLOXs related to the adaptation of radish to stress conditions.