Purpose: The aim of this study was to test the antiviral effectivity of potassium peroxymonosulfate (RUBYSTA, KYORIN) against five epidemic keratoconjunctivitis-related types of in vitro.
Methods: Five types of (8, 37, 53, 54 and 56) were incubated with 1% potassium peroxymonosulfate, 0.1% sodium hypochlorite (NaClO) or alcohol-based disinfectant for 30 s or 1 min. These solutions were subjected to measurements of viral titres by infection assays in A549 cells. At day 6 post-infection, both, supernatants and cells, were collected and the viral genome was assessed by real-time polymerase chain reaction analysis.
Results: Treatments with 1% potassium peroxymonosulfate led to significant reduction in all tested types comparable to disinfecting effects by 0.1% NaClO. Overall, potassium peroxymonosulfate demonstrated sufficient inactivation of the major epidemic keratoconjunctivitis-causing to be considered for disinfection and prevention purposes in ophthalmological clinics and hospitals.
Conclusion: This study demonstrated that potassium peroxymonosulfate is a promising disinfectant for the prevention of epidemic keratoconjunctivitis nosocomial infections in ophthalmological clinics.
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