AI Article Synopsis
- The study focuses on a novel bacteriophage called KP8, which targets a common pathogen known for causing a variety of infections and exhibiting multiple antibiotic resistances.
- KP8 belongs to the Podoviridae family, has a genome size of 73,679 base pairs, and features 97 potential open reading frames, indicating its complexity.
- Comparative and phylogenetic analyses suggest that KP8 has unique evolutionary characteristics that may classify it as a new genus within the N4-like phages, differentiating it from similar viral types.
Article Abstract
is a common pathogen, associated with a wide spectrum of infections, and clinical isolates of often possess multiple antibiotic resistances. Here, we describe a novel lytic N4-like bacteriophage KP8, specific to , including its genome, partial structural proteome, biological properties, and proposed taxonomy. Electron microscopy revealed that KP8 belongs to the Podoviridae family. The size of the KP8 genome was 73,679 bp, and it comprised 97 putative open reading frames. Comparative genome analysis revealed that the KP8 genome possessed the highest similarity to the genomes of and phages, which are N4-like podoviruses. In addition, the KP8 genome showed gene synteny typical of the N4-like podoviruses and contained the gene encoding a large virion-encapsulated RNA polymerase. Phylogenetic analysis of the KP8 genome revealed that the KP8 genome formed a distinct branch within the clade, which included the members of and genera besides KP8. The average evolutionary divergences KP8/ and KP8/ were 0.466 and 0.447 substitutions per site (substitutes/site), respectively, similar to that between and genera (0.468 substitutes/site). The obtained data suggested that phage KP8 differs from other similar phages and may represent a new genus within the N4-like phages.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6950046 | PMC |
| http://dx.doi.org/10.3390/v11121115 | DOI Listing |
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Article Synopsis
- The study focuses on a novel bacteriophage called KP8, which targets a common pathogen known for causing a variety of infections and exhibiting multiple antibiotic resistances.
- KP8 belongs to the Podoviridae family, has a genome size of 73,679 base pairs, and features 97 potential open reading frames, indicating its complexity.
- Comparative and phylogenetic analyses suggest that KP8 has unique evolutionary characteristics that may classify it as a new genus within the N4-like phages, differentiating it from similar viral types.
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