Incidents of food fraud have occurred worldwide, particularly in the form of meat adulteration. In this study, molecular probes were developed using the Random amplification of polymorphic DNA (RAPD) polymerase chain reaction (PCR) technique in order to identify three beef subspecies-Holstein, Angus, and Taiwan Yellow Cattle. Four RAPD-PCR 10-nucleotide primers were chosen out of a total of 60 primers. The selection was based on the reproducibility of species-specific amplicons able to detect various origins of cattle breeds. The results demonstrated that primer OPK12 produced three unique amplicons (1100 bp, 1000 bp and 480 bp) in Holstein; primer OPK14 generated one amplicon that only appeared in Holstein and Angus (200 bp); primer OPK19 amplified two species-specific amplicons in Holstein measuring 550 bp and 650 bp, respectively. However, due to the relatively lower repeatability of RAPD-PCR, higher and more specific testing repeats were required to increase the accuracy of the conclusion.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6859182 | PMC |
| http://dx.doi.org/10.1007/s10068-019-00607-7 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Combined Use of Univariate and Multivariate Approaches to Detect Selection Signatures Associated with Milk or Meat Production in Cattle.
Genes (Basel)
November 2024
Dipartimento di Agraria, Università degli Studi di Sassari, 07100 Sassari, Italy.
Objectives: The aim of this study was to investigate the genomic structure of the cattle breeds selected for meat and milk production and to identify selection signatures between them.
Methods: A total of 391 animals genotyped at 41,258 SNPs and belonging to nine breeds were considered: Angus (N = 62), Charolais (46), Hereford (31), Limousin (44), and Piedmontese (24), clustered in the Meat group, and Brown Swiss (42), Holstein (63), Jersey (49), and Montbéliarde (30), clustered in the Milk group. The population stratification was analyzed by principal component analysis (PCA), whereas selection signatures were identified by univariate (Wright fixation index, F) and multivariate (canonical discriminant analysis, CDA) approaches.
Effects of implant status and breed type on feedlot performance, carcass characteristics, sera metabolites, and immunohistochemical responses in finishing steers.
J Anim Sci
January 2025
Department of Animal and Food Sciences, Texas Tech University, Lubbock, TX USA.
The number of beef × dairy animals entering feedlots has increased, but the response of beef × dairy cattle to growth-promoting implants has not been well characterized. The objective of this study was to evaluate the effects of breed type and implant administration on live performance, carcass characteristics, sera metabolites, and immunohistochemical (IHC) outcomes. Forty-eight steers (average body weight [BW] = 417±22 kg) were sorted by breed into groups of predominantly Angus (B), black-hided beef × primarily Holstein (B×D), or Holstein (D), and half of the steers within each breed type were administered a steroidal implant.
View Article and Find Full Text PDFFactors influencing calving difficulty and gestation length in dairy cows inseminated with beef sires.
Animal
December 2024
Institute of Animal Breeding and Husbandry, Christian-Albrechts-University Kiel, Hermann-Rodewald-Straße 6, 24118 Kiel, Germany.
Producing calves with higher economic value by utilising semen from beef breeds in dairy herds (Beef-on-Dairy, BoD) has become more common in recent times. Such crossbreed calves promise better carcass conformation and higher consumer acceptance due to the better flavour, but the widespread adaptation of BoD requires careful consideration of the sire breed to maximise the benefits. In this regard, two major traits of interest are calving difficulty and gestation length due to associated costs.
View Article and Find Full Text PDFImmortalized mammosphere-derived epithelial cells retain a bioactive secretome with antimicrobial, regenerative, and immunomodulatory properties.
Stem Cell Res Ther
November 2024
Baker Institute for Animal Health, College of Veterinary Medicine, Cornell University, 235 Hungerford Hill Road, Ithaca, NY, 14853, USA.
Background: The secretome of primary bovine mammosphere-derived epithelial cells (MDECs) has been shown to exert antimicrobial, regenerative, and immunomodulatory properties in vitro, which warrants its study as a potential biologic treatment with the potential to be translated to human medicine. Currently, the use of the MDEC secretome as a therapy is constrained by the limited life span of primary cell cultures and the decrease of secretome potency over cell passages.
Methods: To address these limitations, early-passage bovine MDECs were immortalized using hTERT, a human telomerase reverse transcriptase.
Dairy farm management factors associated with auction sale price of young dairy calves sold at auction markets in Québec, Canada-A cross-sectional study.
J Dairy Sci
January 2025
Département de sciences cliniques, Faculté de médecine vétérinaire, Université de Montréal, St-Hyacinthe, QC, J2S 2M2, Canada.
Each year ∼150,000 surplus calves are sold at auction markets in Québec, Canada. Surplus calves (male or female not kept in the herd of origin) are sold at a young age, but these animals are at risk of receiving lower quality neonatal care than replacement heifers. Knowledge of factors associated with a higher selling price could help convince farmers to spend more resources in the care surplus calves.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!