AI Article Synopsis
- Diarrhoea in neonatal pigs is a significant cause of mortality, largely caused by RNA viruses, which were studied using Next Generation Sequencing (NGS) across 47 farms in Spain.
- The research found 12 different virus species from 8 genera, with Rotavirus being the most common, while co-infections were frequent, showcasing a variety of RNA viruses in piglet faeces.
- The study highlighted that Rotaviruses are the primary agents of diarrhoea, with Kobuvirus and Sapovirus also involved, and revealed significant genetic diversity that adds to the understanding of these viruses in the context of enteric diseases.
Article Abstract
Background: Diarrhoea is a major cause of death in neonate pigs and most of the viruses that cause it are RNA viruses. Next Generation Sequencing (NGS) deeply characterize the genetic diversity among rapidly mutating virus populations at the interspecific as well as the intraspecific level. The diversity of RNA viruses present in faeces of neonatal piglets suffering from diarrhoea in 47 farms, plus 4 samples from non-diarrhoeic piglets has been evaluated by NGS. Samples were selected among the cases submitted to the Veterinary Diagnostic Laboratories of Infectious Diseases of the Universitat Autònoma de Barcelona (Barcelona, Spain) and Universidad de León (León, Spain).
Results: The analyses identified the presence of 12 virus species corresponding to 8 genera of RNA viruses. Most samples were co-infected by several viruses. Kobuvirus and Rotavirus were more commonly reported, with Sapovirus, Astrovirus 3, 4 and 5, Enterovirus G, Porcine epidemic diarrhoea virus, Pasivirus and Posavirus being less frequently detected. Most sequences showed a low identity with the sequences deposited in GenBank, allowing us to propose several new VP4 and VP7 genotypes for Rotavirus B and Rotavirus C.
Conclusions: Among the cases analysed, Rotaviruses were the main aetiological agents of diarrhoea in neonate pigs. Besides, in a small number of cases Kobuvirus and Sapovirus may also have an aetiological role. Even most animals were co-infected in early life, the association with enteric disease among the other examined viruses was unclear. The NGS method applied successfully characterized the RNA virome present in faeces and detected a high level of unreported intraspecific diversity.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6896758 | PMC |
| http://dx.doi.org/10.1186/s12917-019-2204-2 | DOI Listing |
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