Various amphiphiles including surfactants and lipids have been designed and synthesized to improve and create new functionalities. In particular, the emergence of cell-like behaviors of giant vesicles (GVs) composed of synthetic lipids has drawn much attention in the development of chemical models for cells. The aim of this study was to measure temperature-dependent morphological changes of GVs induced by fragmentation and subsequent growth using hydrolysable cationic lipids having an amide linkage. Results from differential scanning calorimetry, fluorescence spectroscopy using an environment-responsive probe, and confocal Raman microscopy showed that the dynamics observed were due to changes in the vesicle membrane, including variation in the lipid composition, induced by thermal stimulation.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1021/acs.langmuir.9b02707 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Influence of the glycocalyx on the size and mechanical properties of plasma membrane-derived vesicles.
Soft Matter
December 2024
Robert Frederick Smith School of Chemical and Biomolecular Engineering, Cornell University, Ithaca, NY 14853, USA.
Recent studies have reported that the overexpression of MUC1 glycoproteins on cell surfaces changes the morphology of cell plasma membranes and increases the blebbing of vesicles from them, supporting the hypothesis that entropic forces exerted by MUC1 change the spontaneous curvature of cell membranes. However, how MUC1 is incorporated into and influences the size and biophysical properties of plasma-membrane-blebbed vesicles is not understood. Here we report single-vesicle-level characterization of giant plasma membrane vesicles (GPMVs) derived from cells overexpressing MUC1, revealing a 40× variation in MUC1 density between GPMVs from a single preparation and a strong correlation between GPMV size and MUC1 density.
View Article and Find Full Text PDFAdaptive metal ion transport and metalloregulation-driven differentiation in pluripotent synthetic cells.
Nat Chem
December 2024
Institute of Physiological Chemistry and Pathobiochemistry, University of Münster, Münster, Germany.
Pluripotent cells can yield different cell types determined by the specific sequence of differentiation signals that they encounter as the cell activates or deactivates functions and retains memory of previous inputs. Here, we achieved pluripotency in synthetic cells by incorporating three dormant apo-metalloenzymes such that they could differentiate towards distinct fates, depending on the sequence of specific metal ion transport with ionophores. In the first differentiation step, we selectively transported one of three extracellular metal ion cofactors into pluripotent giant unilamellar vesicles (GUVs), which resulted in elevation of intracellular pH, hydrogen peroxide production or GUV lysis.
View Article and Find Full Text PDFFlow Cytometric Analysis for Evaluating Protein Synthesis Efficiency in Giant Unilamellar Vesicles with Charged Lipids.
Chembiochem
December 2024
University of Fukui: Fukui Daigaku, Department of Applied Chemistry and Biotechnology, 3-9-1 Bunkyo, 910-8507, Fukui-shi, JAPAN.
Quantitative investigation of the relationship between endosomal translation reactions and phospholipid membrane composition is crucial for enhancing protein translation efficiency in artificial cells. In this study, we quantitatively compared the translation reactions within liposomes containing negatively and positively charged lipids using green fluorescent protein fluorescence as an indicator to investigate whether lipid membrane charge affects translation reaction efficiency in artificial cells. Thus, translation efficiency reduced in liposomes containing both negatively and positively charged lipids.
View Article and Find Full Text PDFControlled Lipid Domain Positioning and Polarization in Confined Minimal Cell Models.
Angew Chem Int Ed Engl
December 2024
Ecole Normale Supérieure, Department of Chemistry, 24, rue Lhomond, 75005, Paris, FRANCE.
Giant unilamellar vesicles (GUVs) are widely used minimal cell models where essential biological features can be reproduced, isolated and studied. Although precise spatio-temporal distribution of membrane domains is a process of crucial importance in living cells, it is still highly challenging to generate anisotropic GUVs with domains at user-defined positions. Here we describe a novel and robust method to control the spatial position of lipid domains of liquid-ordered (Lo) / liquid-disordered (Ld) phase in giant unilamellar vesicles (GUVs).
View Article and Find Full Text PDFUnlabelled: Development of an understanding of membrane nanodomains colloquially known as "lipid rafts" has been hindered by a lack of pharmacological tools to manipulate rafts and protein affinity for rafts. We screened 24,000 small molecules for modulators of the affinity of peripheral myelin protein 22 (PMP22) for rafts in giant plasma membrane vesicles (GPMVs). Hits were counter-screened against another raft protein, MAL, and tested for impact on raft, leading to two classes of compounds.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!