AI Article Synopsis
- The legalization of marijuana in multiple states has created challenges for law enforcement regarding driving and machinery operation standards.
- Researchers utilized UV-vis spectroscopy to analyze fingerprints from marijuana users, aiming to detect Δ-THC and its metabolite THC-COOH in a competitive immunoassay.
- The assay involves competition between the THC-COOH metabolite and an enzyme-labeled conjugate for antibody binding, where higher concentrations of the metabolite result in decreased absorbance due to less binding of the conjugate.
Article Abstract
The sudden increase in states legalizing marijuana has forced law enforcement into a situation where the use and consumption are legal, but there are no limitations for what is acceptable for driving or operating machinery. Using ultraviolet-visible (UV-vis) spectroscopy, fingerprints from volunteers who had used marijuana were analyzed via a competitive immunoassay for the detection of Δ-tetrahydrocannabinol (Δ-THC), the main psychoactive component of marijuana, and 11-nor-9-carboxy-THC (THC-COOH), one of the main metabolites produced in the body following the use/consumption of THC-related products. In this research, the THC-COOH metabolite and the enzyme-labeled conjugate compete against each other as the antigens for the system. The antibody used in this assay has a greater affinity for the metabolite; so, as its concentration increases, the absorbance of the system decreases due to less binding of the enzyme-labeled conjugate.
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| http://dx.doi.org/10.1021/acssensors.9b02020 | DOI Listing |
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