Molybdenum oxide quantum dots (MoO QDs) were synthesized by a one-pot method and used as a versatile probe in an electrochemiluminescent (ECL) immunoassay of the non-small cell lung cancer biomarker cytokeratin 19 fragment 21-1 (CYFRA21-1) as a model analyte. The MoO QDs exhibited stable and strong cathodic green ECL, with an emission peak at 535 nm, in the presence of KSO within the potential range of -2.0 to 0 V. On exposure to CYFRA21-1, the ECL decreases because of the immunoreaction between CYFRA21-1 and its antibody which generates a barrier for electron transfer. The determination of CYFRA21-1 with favorable analytical performances was successfully realized under the optimal conditions. ECL decreases linearly in the 1 pg mL to 350 ng mL CYFRA21-1 concentration range, and the detection is as low as 0.3 pg mL. Excellent recoveries from CYFRA21-1-spiked human serum indicate that the assay can be operated under physiological conditions. Graphical abstractSchematic representation of the fabrication of molybdenum oxide quantum dots (MoO QDs) and the electrochemiluminescent (ECL) immunoassay based on the use of the MoO QDs ECL probe for cytokeratin 19 fragment 21-1 (CYFRA21-1).
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