AI Article Synopsis
- Age-related macular degeneration (AMD) is a serious eye disease that can lead to blindness, and it is linked to oxidative stress in the retina.
- The study focuses on how oxidative conditions, such as exposure to hydrogen peroxide, affect the release of small extracellular vesicles (sEVs) from ARPE-19 cells, specifically their miRNA content.
- Findings reveal that while 218 miRNAs were detected in the sEVs, only miR-302a and miR-122 showed significant under-expression under oxidative stress, suggesting a connection between these miRNAs and neovascularization in AMD.
Article Abstract
Age related macular degeneration (AMD) is a common retina-related disease leading to blindness. Little is known on the origin of the disease, but it is well documented that oxidative stress generated in the retinal pigment epithelium and choroid neovascularization are closely involved. The study of circulating miRNAs is opening new possibilities in terms of diagnosis and therapeutics. miRNAs can travel associated to lipoproteins or inside small Extracellular Vesicles (sEVs). A number of reports indicate a significant deregulation of circulating miRNAs in AMD and experimental approaches, but it is unclear whether sEVs present a significant miRNA cargo. The present work studies miRNA expression changes in sEVs released from ARPE-19 cells under oxidative conditions (i.e. hydrogen peroxide, HO). HO increased sEVs release from ARPE-19 cells. Moreover, 218 miRNAs could be detected in control and HO induced-sEVs. Interestingly, only two of them (hsa-miR-302a and hsa-miR-122) were significantly under-expressed in HO-induced sEVs. Results herein suggest that the down regulation of miRNAs 302a and 122 might be related with previous studies showing sEVs-induced neovascularization after oxidative challenge in ARPE-19 cells.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6884596 | PMC |
| http://dx.doi.org/10.1038/s41598-019-54373-x | DOI Listing |
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