The Rapid Carbapenemase Detection Method (rCDM) for Rapid and Accurate Detection of Carbapenemase-Producing and .

This study aimed to design a new method for rapid and accurate detection of carbapenemase phenotypes based on the simplified carbapenem inactivation method (sCIM). We evaluated the sensitivity and specificity of the method, called the rapid carbapenemase detection method (rCDM), for the detection of carbapenemase-producing and . A total of 257 , 236 , and 20 isolates were tested. Phenotypic evaluations were performed using rCDM, sCIM, and mCIM. For , the sensitivity of rCDM was 100% and the specificity was 99.6%. For . , the sensitivity of rCDM was 97.4% and the specificity was 100%. Carbapenemase-producing were not detected by rCDM. The concordance rate of rCDM and sCIM for and was 99.8%, with the exception of one isolate that expressed the gene. The concordance rate of rCDM and mCIM for and was 100%. rCDM can be used to accurately detect carbapenemase-producing and in 5-6 h and is suitable for routine use in most clinical microbiology laboratories.