This study aimed at detecting Staphylococcus aureus from white coats of college students and characterizing antimicrobial susceptibility and biofilm production. Bacterial samples (n = 300) were obtained from white coats of 100 college students from August 2015 to March 2017 S. aureus was isolated and it´s resistance profile was assessed by antimicrobial disk-diffusion technique, screening for methicillin-resistant Staphylococcus aureus (MRSA), detection of mecA gene by PCR, and determination of staphylococcal cassette chromosome mec (SCCmec) by multiplex PCR. Congo red agar (CRA) and icaA and icaD genes by PCR were used for biofilm characterization. S. aureus was identified in 45.0% of samples. Resistance of S. aureus sample to antimicrobial was seen for penicillin (72.59%), erythromycin (51.85%), cefoxitin (20.74%), oxacillin (17.04%), clindamycin (14.81%) and levofloxacin (5.18%). MRSA was detected in 53.3% of the samples with SCCmec I (52.8%), SCCmec III (25%) and SCCmec IV (11.1%). Biofilm production was observed in 94.0% S. aureus samples. These data show that biosafety measures need to be enhanced in order to prevent dissemination of multiresistant and highly adhesive bacteria across other university settings, relatives, and close persons.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6883624 | PMC |
| http://dx.doi.org/10.1186/s12941-019-0337-6 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Rapid High-Throughput Discovery of Molecules With Antimicrobial Activity From Natural Products Enabled by a Nanoliter Matrix SlipChip.
Small Methods
January 2025
School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, 200030, China.
Improper use of antibiotics has led to the development of antimicrobial resistance, or "superbugs," outpacing the discovery of new antibiotics. The lack of rapid, high-throughput screening methods is a major bottleneck in discovery novel antibiotics. Traditional methods consume significant amounts of samples, making it challenging to discover new antibiotics from limited natural product extracts.
View Article and Find Full Text PDFComparative analyses of persistence traits in O157:H7 strains belonging to different clades including REPEXH01 and REPEXH02 strains.
Front Microbiol
December 2024
Meat Safety and Quality Research Unit, U.S. Department of Agriculture, U.S. Meat Animal Research Center, Clay Center, NE, United States.
Recent application of whole genome sequencing in the investigation of foodborne illness outbreaks has facilitated the identification of Reoccurring, Emerging, or Persistent (REP) bacterial strains that have caused illnesses over extended periods of time. Here, the complete genomes of two O157:H7 (EcO157) outbreak strains belonging to REPEXH01 and REPEXH02, respectively, were sequenced and annotated. Comparative genomics and phenotypic analyses were carried out to identify REP-associated traits.
View Article and Find Full Text PDFExploring water quality variations and biofilm growth in a drinking water distribution system via a biofilm annular reactor series system and predictive modelling of residual chlorine.
Chemosphere
December 2024
NUS Environmental Research Institute, National University of Singapore, 5A Engineering Drive 1, Singapore 117411; Department of Civil & Environmental Engineering, National University of Singapore, 1 Engineering Drive 2, Singapore 117576. Electronic address:
The hydraulic conditions vary significantly across different segments of the drinking water distribution system (DWDS), leading to distinct variations in water quality throughout the system. Understanding these changes in water quality and biofilm development over time is crucial for enhancing drinking water management efficiency. This study focused on replicating the hydraulic conditions found in transmission and distribution pipelines within a specific pipeline path of the DWDS in Singapore using a biofilm annular reactor series system (BARSS).
View Article and Find Full Text PDFGingivitis- and periodontitis-associated microbiota in bovine deciduous incisor teeth - a preliminary study.
Microbes Infect
December 2024
Department of Production and Animal Health, School of Veterinary Medicine, São Paulo State University (Unesp), Araçatuba, São Paulo, Brazil. Electronic address:
As ruminants are frequently affected by periodontal diseases, understanding their microbial communities is crucial. In this pilot study, we analyzed subgingival biofilm samples of young cattle across different states: clinically healthy (n=5), gingivitis (n=5), and periodontitis (n=5) using 16S rRNA gene sequencing and co-occurrence network analysis. The findings revealed that Proteobacteria was the predominant phylum across all conditions, with Fusobacteriota constituting 27.
View Article and Find Full Text PDFClinical aspects and characterization of Pseudomonas aeruginosa isolated from patients infected with SARS-CoV-2.
Microb Pathog
December 2024
Health Sciences, Universidade do Oeste Paulista/UNOESTE Rua José Bongiovani, 700 - Cidade Universitária, CEP: 19050-920. Presidente Prudente, SP Brazil; Program of Animal Science, Universidade do Oeste Paulista/UNOESTE Rua José Bongiovani, 700 - Cidade Universitária, CEP: 19050-920. Presidente Prudente, SP Brazil. Electronic address:
Aims: This study aimed to identify and characterize Pseudomonas aeruginosa isolates from patients infected and uninfected with SARS-CoV-2, focusing on their phenotypic characteristics and antimicrobial resistance profiles.
Main Methods: A total of 100 P. aeruginosa isolates were obtained from patients admitted to a hospital in Presidente Prudente, SP, in 2021.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!