The search for potential partners of human SELM in lysates of two cancer cell lines, HT-1080 (fibrosarcoma) and MCF-7 (breast adenocarcinoma), was carried out. Two cytoplasmic actin isoforms-cytoplasmic actin 1 (cytoskeleton β-actin) and cytoplasmic actin 2 (cytoskeletal γ-actin)-were identified as partners. In addition, the influence of two widely used antitumor selenium compounds (sodium selenite and methylseleninic acid) on the expression of SELM in cancer cells was studied. On the basis of the results obtained by real-time PCR and Western blotting, we concluded that 1 μM and 10 μM sodium selenite did not affect the expression of SELM in fibrosarcoma cells, whereas in breast adenocarcinoma cells 1 μM sodium selenite slightly increased expression and 10 μM sodium selenite significantly (approximately 2 times) decreased it. Methylseleninic acid in both cancer cell lines increased the SELM gene expression; the most pronounced effect was observed when fibrosarcoma cells were treated with 10 µM MSA (the expression of the hSelm gene increased almost 4 times).
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1134/S1607672919050065 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Sagittaria sagittifolia polysaccharide extract regulates Nrf2 to improve endoplasmic reticulum stress-mediated apoptosis in rat cataracts and HLEB3 cells.
Int J Biol Macromol
January 2025
School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 102446, China. Electronic address:
Age-related cataract (ARC) remains the leading cause of blindness worldwide. Sagittaria sagittifolia polysaccharide (SSP) extract, a key component of Sagittaria sagittifolia L., exhibits anti-oxidant and anti-apoptotic effects with potential applications in ARC.
View Article and Find Full Text PDFSelenium-Chondroitin Sulfate Nanoparticles Inhibit Angiogenesis by Regulating the VEGFR2-Mediated PI3K/Akt Pathway.
Mar Drugs
January 2025
College of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, China.
Chondroitin sulfate (CS), a class of glycosaminoglycans covalently attached to proteins to form proteoglycans, is widely distributed in the extracellular matrix and cell surface of animal tissues. In our previous study, CS was used as a template for the synthesis of seleno-chondroitin sulfate (SeCS) through the redox reaction of ascorbic acid (Vc) and sodium selenite (NaSeO) and we found that SeCS could inhibit tumor cell proliferation and invasion. However, its effect on angiogenesis and its underlying mechanism are unknown.
View Article and Find Full Text PDFSelenium enrichment enhances the alleviating effect of GG on alcoholic liver injury in mice.
Curr Res Food Sci
December 2024
Department of Hepatopancreatobiliary Surgery, Fujian Research Institute of Abdominal Surgery, The First Affiliated Hospital of Fujian Medical University, Fuzhou, Fujian, 350005, PR China.
Selenium-enriched probiotics have attracted much attention due to the physiological activities of both probiotics and selenium (organic selenium). In this study, we investigated the mitigating effect of selenium-enriched GG (LGG@Se) and its pathway on alcohol-induced liver injury (ALI) in mice. The results showed that LGG@Se was superior to LGG and sodium selenite in alleviating ALI.
View Article and Find Full Text PDFDate seeds polysaccharides as novel capping agents for selenium nanoparticles: Synthesis, characterization, stability, biological activities, and gut microbiota modulation.
Food Chem
January 2025
Department of Food Science, College of Agriculture and Veterinary Medicine, United Arab Emirates University (UAEU), PO Box 15551, Al-Ain, United Arab Emirates. Electronic address:
Date seed polysaccharides were utilized to synthesize selenium nanoparticles (MPS-NP) through a redox reaction involving sodium selenite and ascorbic acid. Characterization of MPS-NP showed a uniform, amorphous, spherical shape with a particle size of 89.2 nm, remaining stable for 42 days.
View Article and Find Full Text PDFSodium selenite enhanced the selenium content in black soldier fly.
Sci Rep
January 2025
College of Animal Science, Anhui Science and Technology University, Bengbu, 233100, People's Republic of China.
This study focuses on the effects of different levels of sodium selenite on the growth, selenium content, and antioxidant capacity of black soldier fly (Hermetia illucens). The experiment used different doses of sodium selenite for treatment, including a basic diet with no supplements (control) and diets supplemented with 10 mg/kg (Se10), 20 mg/kg (Se20), 30 mg/kg (Se30), and 40 mg/kg (Se40) sodium selenite, and results show that sodium selenite supplementation significantly increases selenium content and improves selenium utilization and antioxidant capacity (P < 0.05).
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!