The present manuscript reports the synthesis and characterization of polymerizable ammonium-based ionic liquids (ILs) and explores their influence on structural stability of stem bromelain (BMN) by using various biophysical techniques. Thermal fluorescence results showed that N-ethyl-2-(methylacryloyloxy)-N,N-dimethylethan-1-ammonium bromide (IL) (at lower concentration (0.1 mg/mL)) is found to be increasing the thermal stability of BMN which can be evident from the transition temperature (T) for BMN in IL (68.51 °C) is higher than BMN in buffer (66.24 °C). Whereas, N-(2-(methacryloyloxy)ethyl)-N,N-dimethylpropan-1-ammonium bromide (IL) and N-(2-(methacryloyloxy)ethyl)-N,N-dimethylpentan-1-ammonium bromide (IL) are maintaining the T values very near to T of pure BMN. Though, N-(2-(methacryloyloxy)ethyl)-N,N-dimethylhexan-1-ammonium bromide (IL) is found to be destabilizing IL as it significantly decreased the T value of BMN at all concentrations. Additionally, consequence of ILs on the proteolytic activity of BMN has also performed for IL up to 5 mg/mL while IL and IL at 0.1 mg/mL and 0.5 mg/mL are enhancing the proteolytic activity of BMN. Later, molecular docking studies are also performed with AutoDock vina and results showed that all ILs have different binding sites on BMN however IL is observed to be binding to the catalytic site of the BMN, it turns out to be the most destabilizing IL.
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