infection in honeybee samples from Tuscanian Archipelago (Central Italy) investigated by two qPCR methods.

and are microsporidian parasite worldwide spread causing an emerging infectious disease of European honeybee The presence was deeply investigated in several countries but low information are presents about islands. In this investigation was evaluated the presence and in apiaries located in Tuscanian Archipelago islands (Central Italy). For detection, two different Real-Time PCR (qPCR) methods, the rRNA and gene amplification qPCR, were performed on honey bee samples; while, for only the rRNA qPCR amplification was performed. On all islands, only was present, while was not found in the samples The two qPCR showed significant difference (p < 0.040) in spores quantification. The single-copy gene method qPCR assay systematically detected a lower amount of copies compared to the multi-copy gene method.