AI Article Synopsis
- Prodigiosin production in Serratia marcescens AS-1 is regulated by quorum sensing, which increases with higher levels of N-hexanoyl homoserine lactone (C6HSL).
- When combined with C6HSL, immobilized cyclodextrin (CD) can form inclusion complexes that reduce prodigiosin production significantly.
- Additionally, CD-immobilized gels can inhibit the bacteria's swarming motility, suggesting they may serve as effective materials for preventing bacterial infections.
Article Abstract
Prodigiosin production in the model opportunistic human pathogen, Serratia marcescens AS-1, depends on quorum sensing (QS), the activation of which is induced by an increase in N-hexanoyl homoserine lactone (C6HSL) concentration. When mixed with C6HSL in the broth culture of S. marcescens immobilized cyclodextrin (CD) effectively formed an inclusion complex resulting in suppression of the QS-mediated prodigiosin production. Thousands of parallel gel filament bundles could be fabricated by the dynamic flow of aqueous sodium alginate blended with α-CD or hydroxypropyl-β-CD (HP-β-CD) immobilized hydroxypropyl cellulose (HPC) inside a co-axial microfluidic device. Shear-induced fibrous domains of HPC-alginate complex assembly were immediately jacketed by calcium alginate gel formed by a sheath flow containing Ca ions. Immobilized α-CD and HP-β-CD on the HPC/alginate gel fibers could inhibit the C6HSL-mediated prodigiosin production to approximately 10%. Furthermore, the swarming motility of S. marcescens AS-1 was effectively suppressed in the presence of free or immobilized α-CD or HP-β-CD. The CD-immobilized gel filament bundle is a novel type of preventive material against bacterial infections.
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| http://dx.doi.org/10.1016/j.msec.2019.110331 | DOI Listing |
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