Phylo-diversity of prevalent human O157:H7 with strains from retailed meat and fish in selected markets in Ibadan Nigeria.

Global prevalence of ESBL-biotypes poses a serious threat to public health as a result of severity and morbidity caused by beta-lactam encoded . Therefore, the prevalent shiga toxigenic of ESBL variant was investigated in various retailed food animals and cooking materials. A total of 823 samples consisting of raw meat (297) and fish (132) samples retailed at various major markets in Ibadan were collected and 394 swabs were taken from the butchers' processing tables and utensils used in retailing meat and fish. The samples were cultured and biotyped for . Serological and PCR assay were used to identify O157:H7 variant and antibiotics resistant determinants. Genetic relatedness of characterized O157 from human and meat products was evaluated with phylogenetic analysis. Of all the isolates, 130 (15.8%) were and only 8 (1.0) were O157:H7 while 4 (50%) were resistant to one or more antibiotics with resistance index ranging from 0.1 to 0.5. More than 25% O157:H7 were resistant to Ampicillin, Pefloxacin and Gentamicin and bla and bla were detected in 1/8 (12.5%) of O157:H7 and bla 3/8 (37.5%) respectively. Only 1 genotyped human .0157:H7 clustered with beef strain There is evidence of TEM encoded O157:H7 causing infection in human from food animals retailed in many markets within various communities. Therefore, urgent surveillance with public health education, food, and environmental hygiene are highly needed to prevent its spread.