A new "turn-on" fluorescence probe based on hydrazine-triggered tandem reaction.

Spectrochim Acta A Mol Biomol Spectrosc

Key Laboratory of Medicinal Chemistry and Molecular Diagnosis of the Ministry of Education, Key Laboratory of Chemical Biology of Hebei Province, College of Chemistry & Environmental Science, Hebei University, Baoding 071002, PR China. Electronic address:

Published: March 2020

Hydrazine is extremely harmful to the human body. The leakage of hydrazine is liable to cause potential safety hazards. Here, we reported a new fluorescence probe based on the tandem reaction. The hydrazine-triggered hydrazinolysis-cyclization resulted in the formation of the iminocoumarin. The fluorescence intensity at 522 nm of the probe increased after the reaction with hydrazine. There was a linear relationship between the fluorescence intensity and the concentration of hydrazine (0.14-120.00 μM). The LOD of the probe to NH was 1.36 ppb. Notably, the probe could detect hydrazine in BT474 cells and tap water.

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http://dx.doi.org/10.1016/j.saa.2019.117754DOI Listing

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