This study was conducted to validate a simulated commercial baking process for plain muffins against E. coli O121 (isolated from the recent illness outbreak associated with flour), and compare the thermal inactivation parameters (D- and z-values) of cocktails of four isolates of E. coli O121 and three serovars of Salmonella (Newport, Typhimurium, and Senftenberg) in muffin batter. Flour samples were spray inoculated with the E. coli O121 or Salmonella cocktails, dried back to the pre-inoculation weight to achieve ~7 log CFU/g, and used to prepare muffin batter. For the muffin baking validation study using E. coli O121, muffin batter was baked at 375 °F (190.6 °C) oven temperature for 21 min followed by 30 min of ambient cooling. The E. coli O121 population decreased by >7 log CFU/g in muffins by 17 min of baking, and was completely eradicated after 21 min of baking and ambient cooling. The D-values of E. coli O121 and Salmonella cocktails in muffin batter at 60, 65 and 70 °C were 42.0 and 38.4, 7.5 and 7.2, and 0.4 and 0.5 min, respectively; whereas the z-values of E. coli O121 and Salmonella were 5.0 and 5.2 °C, respectively.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.ijfoodmicro.2019.108422 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Hydrolysis probe assays for the detection of pathogenic Enterohemorrhagic Escherichia coli: Multi-Country validation study.
Food Res Int
November 2024
Department of Health, Nutrition and Food Sciences, Florida State University, Tallahassee, FL, USA. Electronic address:
Article Synopsis
- Enterohemorrhagic Escherichia coli (EHEC) strains, linked to red meat outbreaks, pose serious global food safety issues, especially since current detection methods often yield false positives due to non-pathogenic strains.
- This study focused on developing and validating four new hydrolysis probe assays specifically designed to detect pathogenic E. coli serogroups O26, O103, O111, and O121, showing an impressive accuracy of 94.8% in identifying virulent strains.
- The newly standardized assays offer reliable detection tools for food laboratories worldwide, helping to mitigate waste and financial losses from incorrect positive test results associated with harmless non-pathogenic E. coli.
Overview of pathogenic Escherichia coli, with a focus on Shiga toxin-producing serotypes, global outbreaks (1982-2024) and food safety criteria.
Gut Pathog
October 2024
Saudi Food and Drug Authority, Riyadh, Saudi Arabia.
Classification of pathogenic E. coli has been focused either in mammalian host or infection site, which offers limited resolution. This review presents a comprehensive framework for classifying all E.
View Article and Find Full Text PDFEnteric pathogen survival, food safety incidents, and potential mitigation strategies to address microbial contamination in wheat-based foods: a review.
Crit Rev Food Sci Nutr
August 2024
Department of Grain Science and Industry, Kansas State University, Manhattan, KN, USA.
Wheat-based foods has emerged as another potential vehicle for foodborne illness in humans. The recent occurrence of recalls involving wheat-based foods requires a full understanding of how these pathogens thrive in these food products and developing potential intervention strategies to address pathogen contamination. This manuscript is the second of a two-part review covering the status of the food safety of wheat-based products.
View Article and Find Full Text PDFSample Type and Processing Plant Differences in the Proportion of Enterohemorrhagic O157 and Non-O157 Serogroups in Feces and on Hides of Cull Dairy Cattle at Slaughter.
Foodborne Pathog Dis
November 2024
Center for Outcomes Research and Epidemiology, College of Veterinary MediciMine, Kansas State University, Manhattan, Kansas, USA.
The study was conducted to determine the proportion and concentration of enterohemorrhagic (EHEC) O157 and six non-O157 (O26, O45, O103, O111, O121, and O145) serogroups and identify seasonal and processing plant differences in feces and on hides of cull dairy cattle processed in commercial slaughterhouses in the United States. Approximately 60 rectal and 60 hide-on samples from matched carcasses were collected in each of three processing plants, in two periods; summer of 2017 and spring of 2018. Samples before enrichment were spiral plated to quantify EHEC, and postenriched samples underwent culture methods that included immuno-magnetic separation, plating on selective media, and PCR assays for identification and serogroup confirmation of putative isolates.
View Article and Find Full Text PDFThe Use of Antimicrobial Washes to Inactivate Shiga Toxin-Producing Escherichia coli from In-Shell Pecans and Wash Water Contaminated by Different Inoculation Routes.
J Food Prot
September 2024
Department of Food Science, University of Arkansas System Division of Agriculture, 2650 N Young Ave., Fayetteville, AR 72704, USA. Electronic address:
In-shell pecans are typically harvested after falling from trees to the ground, presenting a potential route of contamination of foodborne pathogens from soil contact. In-shell pecans are often subjected to various processing or washing steps prior to being shelled. This study determined Shiga toxin-producing Escherichia coli (STEC) reductions after treatment with antimicrobial washes on direct and soil-inoculated in-shell pecans and evaluated the cross-contamination potential of the spent pecan washes after treatment.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!