A novel bgl1 gene, encoding GH3 family β-glucosidase from Penicillium verruculosum (PvBGL), was cloned and heterologously expressed in P. canescens RN3-11-7 (niaD-) strain under the control of the strong xylA gene promoter. The recombinant rPvBGL was purified and their properties were studied in comparison with those of rAnBGL from Aspergillus niger expressed previously in the same fungal host. The rPvBGL had an observed molecular mass of 90 kDa (SDS-PAGE data) and displayed the enzyme maximum activity at pH 4.6 and 65 °C. The enzyme half-life time at 60 °C was found to be 87 min. Unlike the rAnBGL, the rPvBGL was not adsorbed on microcrystalline cellulose, which gives the latter enzyme an advantage in cellulose conversion with a longer time of hydrolysis.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.biochi.2019.11.009 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Commentary on: "Structural insights into a bacterial β-glucosidase capable of degrading sesaminol triglucoside to produce sesaminol: toward the understanding of the aglycone recognition mechanism by the C-terminal lid domain".
J Biochem
January 2025
Department of Molecular Immunology, Research Institute for Microbial Diseases.
Sesaminol is an organic compound which shows the strong antioxidant, anti-inflammatory, and neuroprotective properties. Sesaminol triglucoside (STG) is glycosylated form of sesaminol and abundantly exists in sesame seeds. However, typical β-glucosidases could not deglycosylate STG probably due to its bulky aglycone.
View Article and Find Full Text PDFTranscriptome Analysis Provides Insights into the Safe Overwintering of Local Peach Flower Buds.
Curr Issues Mol Biol
December 2024
Institute of Fruit and Floriculture Research, Gansu Academy of Agricultural Sciences, Anning, Lanzhou 730070, China.
During the dormant period of peach trees in winter, flower buds exhibit weak cold resistance and are susceptible to freezing at low temperatures. Understanding the physiological and molecular mechanisms underlying the response of local peach buds to low-temperature adversity is crucial for ensuring normal flowering, fruiting, and yield. In this study, the experimental materials included the conventional cultivar 'Xia cui' (XC) and the cold-resistant local resources 'Ding jiaba' (DJB) peach buds.
View Article and Find Full Text PDFSignal transducer of IAA related gene expression induces transporters of hyperaccumulator for Pb accumulation.
Int J Phytoremediation
December 2024
College of Resources and Environment, Yunnan Agricultural University, Kunming, P.R. China.
Lead (Pb) pollution in soil affects growth of plants. Plants' endogenous hormones play an important role in resistance to Pb of plant. In order to explore the hormone-based mechanisms of Pb accumulationin in hyperaccumulator , a pot experiment was conducted to analyze the contents of endogenous hormones (auxin, gibberellin, abscisic acid, and cytokinin) and related genes expressions, and Pb contents of , as well as the transporter (cation exchangers (CAX), heavy metal ATPases (HMA), and ATP-binding cassette (ABC)) concentrations under foliar spraying of indoleacetic acid (IAA).
View Article and Find Full Text PDFStructural and functional insights into recombinant β-glucosidase from Thermothelomyces thermophilus: Cello-oligosaccharide hydrolysis and thermostability.
Enzyme Microb Technol
December 2024
Department of Biotechnology, Lorena School of Engineering, University of São Paulo, Lorena, SP, Brazil. Electronic address:
β-glucosidases (BGLs) are key enzymes in the depolymerization of cellulosic biomass, catalyzing the conversion of cello-oligosaccharides into glucose. This conversion is pivotal for enhancing the production of second-generation ethanol or other value-added products in biorefineries. However, the process is often cost-prohibitive due to the high enzyme loadings required.
View Article and Find Full Text PDFGenome-based analysis reveals niche differentiation among Firmicutes in full-scale anaerobic digestion systems.
Bioresour Technol
December 2024
School of Civil, Environmental and Architectural Engineering, Korea University, Seoul, South Korea. Electronic address:
Fermentative Firmicutes species are key players in anaerobic digestion; however, their niche differentiation based on carbohydrate utilization in full-scale systems remains unclear. In this study, we investigated niche differentiation among four major Firmicutes classes using a genome-centric approach, reconstructing 39 high-quality metagenome-assembled genomes. Limnochordia and Clostridia exhibited the broadest substrate versatility, utilizing 24% and 18% of the predicted substrates, respectively.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!