sp. nov., a novel alkaliphilic bacterium from An Indian Soda Lake.

A novel Gram-stain-negative bacterial strain designated as MEB193 was isolated from a sediment sample collected from Lonar Lake, India. The cells were motile, non-spore-forming and rod-shaped. The strain was oxidase- and catalase-positive. It grew optimally at pH 9.0 and at 1 % (w/v) NaCl concentration at 30 °C. Based on 16S rRNA gene sequence similarity, MEB193 belongs to genus , with ZV-19 (95.89 %) and 4CA (95.87 %) as its closest neighbours. The major fatty acid was summed feature 8 comprising Cω7c/Cω6c (52 %) followed by C (25 %). Phosphatidylglycerol (PG) and diphosphatidylglycerol (DPG) were present as the major polar lipids. The draft genome obtained in this study was 2 793 747 bp and the G+C content was 50.79 mol%. Average nucleotide identity (71.76 %) and DNA-DNA hybridization (<20 %) values between strain MEB193 and 4CA confirmed the novelty of this new species. Based on phenotypic including chemotaxonomic and genotypic characterization data, strain MEB193 represents a new species of the genus for which the name sp. nov. is proposed. The type strain is MEB193 (=MCC 2863=JCM 31570 =KCTC 52390 ).