Design and synthesis of fluorescent probe with fast response, excellent water solubility and good hepatocyte-targeting capacity to detect hydrogen sulfide (HS) in hepatocytes and water samples is of great significance. Here, a novel fluorescent probe QL-Gal-N for detection of HS was designed and synthesized based on HS-mediated azide reduction strategy. This sensor demonstrated low toxicity, fast response (within 1 min), high selectivity and low detection limit (as low as 126 nM in water) for the detection of HS. HeLa, A549 and HepG-2 cells were chosen to investigate the hepatocyte-targeting ability of QL-Gal-N respectively. The results indicated that the specific recognition of ASGPR over-expressed in hepatocytes by galactose group was an important reason for the good targeting ability of probe QL-Gal-N. Furthermore, due to the introduction of glycosyl moiety, the water solubility of fluorescent probe was enhanced obviously. It was successfully applied for the detection of HS in environmental water samples including river water, tap water, lake water and waste water.
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| http://dx.doi.org/10.1016/j.saa.2019.117690 | DOI Listing |
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