Three bacterial strains, HKU70, HKU71 and HKU72, were isolated from the conjunctival swab, blood and sputum samples of three patients with conjunctivitis, bacteraemia and respiratory infection, respectively, in Hong Kong. The three strains were aerobic, Gram-stain positive, catalase-positive, non-sporulating and non-motile bacilli and exhibited unique biochemical profiles distinguishable from currently recognized species. 16S rRNA, , and gene sequence analyses revealed that the three strains shared 99.6-99.9, 94.5-96.8, 95.7-97.8 and 97.7-98.9 % nucleotide identities with their corresponding closest species respectively. DNA-DNA hybridization confirmed that they were distinct from other known species of the genus (26.2±2.4 to 36.8±1.2 % DNA-DNA relatedness), in line with results of genome-to-genome comparison (32.2-40.9 % Genome-to-Genome Distance Calculator and 86.3-88.9 % average nucleotide identity values]. Fatty acids, mycolic acids, cell-wall sugars and peptidoglycan analyses showed that they were typical of members of . The G+C content determined based on the genome sequence of strains HKU70, HKU71 and HKU72 were 69.9, 70.2 and 70.5 mol%, respectively. Taken together, our results supported the proposition and description of three new species, i.e. HKU70 (=JCM 33387=DSM 109106) sp. nov., HKU71 (=JCM 33388=DSM 109107) sp. nov. and HKU72 (=JCM 33389=DSM 109108) sp. nov.
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