Facilitates Apoptosis, ROS Generation, and Inflammatory Cytokine Production by Activating AKT/MAPK and NF-B Signaling Pathways in Human Gingival Fibroblasts.

Oxid Med Cell Longev

Shandong Provincial Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration, School of Stomatology, Shandong University, Jinan, Shandong 250012, China.

Published: May 2020

() plays key roles in the initiation and progression of periodontitis. However, the pathogenic effect of on human oral tissues and cells has not been fully evaluated. In this study, we aimed to analyze the pathogenic effects of on human gingival fibroblasts (GFs) and clarify the potential mechanisms. RNA-sequencing analysis confirmed that significantly altered the gene expression of GF as the stimulation time increased. Cell counting and EdU-labeling assays indicated that inhibited GF proliferation and promoted cell apoptosis in a time- and dose-dependent manner. In addition, cell apoptosis, intracellular reactive oxygen species (ROS) generation, and proinflammatory cytokine production were dramatically elevated after stimulation. Furthermore, we found that the AKT/MAPK and NF-B signaling pathways were significantly activated by infection and that a large number of genes related to cellular proliferation, apoptosis, ROS, and inflammatory cytokine production downstream of AKT/MAPK and NF-B signaling pathways were significantly altered in -stimulated GFs. These findings suggest that inhibits GF proliferation and promotes cell apoptosis, ROS generation, and inflammatory cytokine production partly by activating the AKT/MAPK and NF-B signaling pathways. Our study opens a new window for understanding the pathogenic effects of periodontal pathogens on the host oral system.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6815639PMC
http://dx.doi.org/10.1155/2019/1681972DOI Listing

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