Exploring the Role of Relish on Antimicrobial Peptide Expressions (AMPs) Upon Nematode-Bacteria Complex Challenge in the Nipa Palm Hispid Beetle, Maulik (Coleoptera: Chrysomelidae).

The humoral immune responses of the nipa palm hispid beetle involves the inducible expression of the genes coding for antimicrobial peptides (AMPs) which are mediated by immune deficiency signaling pathways. In insects, the nuclear factor-κB (NF-κB) transcription factor, Relish, has been shown to regulate AMP gene expressions upon microbial infections. Here, we dissect the expression patterns of some AMPs in during infections by entomopathogenic nematodes (EPNs) and their symbionts, before and after Relish knock down. Our results indicate that, prior to gene silencing, the AMPs attacin C1, attacin C2, and defensin 2B were especially expressed to great extents in the insects challenged with the nematodes and as well as with their respective symbionts and . The study also established the partial sequence of OnRelish/NF-κB p110 subunit in , with an open reading frame coding for a protein with 102 amino acid residues. A typical Death domain-containing protein was detected (as seen in ) at the C-terminus of the protein. Phylogenetic analysis revealed that in , Relish is clustered with registered Relish/NF-κB p110 proteins from other species of insect especially from the same order Coleoptera. Injection of OnRelish dsRNA remarkably brought down the expression of OnRelish and also reduced the magnitude of transcription of attacin C1 and defensin 2B upon and and their symbionts infections. Altogether, our data unveil the expression pattern of OnRelish as well as that of some AMP genes it influences during immune responses of against EPNs and their symbionts.