Biosynthesized silver nanoparticles using Bacillus amyloliquefaciens; Application for cytotoxicity effect on A549 cell line and photocatalytic degradation of p-nitrophenol.

J Photochem Photobiol B

Innovative Green Product Synthesis and Renewable Environment Development Research Group, Faculty of Environment and Labour Safety, Ton Duc Thang University, Ho Chi Minh City, Viet Nam. Electronic address:

Published: January 2020

AI Article Synopsis

  • The study successfully synthesized silver nanoparticles (AgNPs) using the cell-free supernatant of Bacillus amyloliquefaciens MSR5, demonstrating their stability and characteristics through various methods.
  • The AgNPs were created via ultrasonic irradiation, showing a spherical shape and size range of 20-40 nm, with strong catalytic activity for degrading 4-nitrophenol (4-NP) under solar light, achieving 98% degradation within 15 minutes.
  • Additionally, cytotoxicity tests indicated that the biosynthesized AgNPs have dose-dependent effects on A549 cells, suggesting potential applications for removing hazardous pollutants from wastewater.

Article Abstract

The present study reports the biosynthesis of silver nanoparticles (AgNPs) using Bacillus amyloliquefaciens MSR5. The cellfree supernatant of B. amyloliquefaciens acted as a stabilizing agent for the synthesis of AgNPs. The synthesized AgNPs were characterized using UV-vis spectrophotometer, PXRD, FTIR, SEM-EDX, DLS, and TEM. TEM image showed the spherical shape of the biosynthesized AgNPs and it was found to be 20-40 nm in range. In this study, the AgNPs were prepared by ultrasonic irradiation. The stability of the AgNPs was found to be -33.4 mV using zeta potential. The catalytic 4-nitrophenol (4-NP) degradation by AgNPs was examined under solar irradiation and furthermore, the effects of several degradation parameters were studied. The biosynthesized AgNPs exhibited a strong chemocatalytic action with a comprehensive degradation (98%) of 4-NP to 4-aminophenol (4-AP) using NaBH within 15 min. In addition, MTT assay was performed to evaluate the cytotoxicity of the biosynthesized AgNPs (10 - 200 μg). The results have shown that the AgNPs exhibited significant activity on A549 cells, which was dosedependent. The study elucidates the AgNPs synthesized using cellfree culture supernatant can be used for the elimination of hazardous pollutants from wastewater.

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http://dx.doi.org/10.1016/j.jphotobiol.2019.111642DOI Listing

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