ROCK inhibitor increases proacinar cells in adult salivary gland organoids.

Stem Cell Res

Graduate program in Molecular, Cellular, Developmental and Neural Biology, University at Albany, State University of New York, Albany, NY 12222, USA; Department of Biological Sciences, University at Albany, State University of New York, Albany, NY 12222, USA; RNA Institute, University at Albany, State University of New York, Albany, NY 12222, USA; Department of Biological Sciences, University at Albany, SUNY, 1400 Washington Ave., LSRB 1086, Albany, NY 12222, USA. Electronic address:

Published: December 2019

AI Article Synopsis

  • Salisphere-derived adult epithelial cells show promise in enhancing saliva production in irradiated mouse salivary glands through optimizations in cellular composition.
  • The ROCK inhibitor Y27632 was tested for its ability to promote the growth and differentiation of salivary epithelial progenitor cells in various media.
  • Results indicated that Y27632 boosted the expansion of specific cell types and improved the presence of markers associated with salivary gland function in salisphere and organoid cultures, supporting its potential in regenerative medicine.

Article Abstract

Salisphere-derived adult epithelial cells have been used to improve saliva production of irradiated mouse salivary glands. Importantly, optimization of the cellular composition of salispheres could improve their regenerative capabilities. The Rho Kinase (ROCK) inhibitor, Y27632, has been used to increase the proliferation and reduce apoptosis of progenitor cells grown in vitro. In this study, we investigated whether Y27632 could be used to improve expansion of adult submandibular salivary epithelial progenitor cells or to affect their differentiation potential in different media contexts. Application of Y27632 in medium used previously to grow salispheres promoted expansion of Kit and Mist1 cells, while in simple serum-containing medium Y27632 increased the number of cells that expressed the K5 basal progenitor marker. Salispheres derived from Mist1; R26 mice grown in salisphere media with Y27632 included Mist1-derived cells. When these salispheres were incorporated into 3D organoids, inclusion of Y27632 in the salisphere stage increased the contribution of Mist1-derived cells expressing the proacinar/acinar marker, Aquaporin 5 (AQP5), in response to FGF2-dependent mesenchymal signals. Optimization of the cellular composition of salispheres and organoids can be used to improve the application of adult salivary progenitor cells in regenerative medicine strategies.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7069099PMC
http://dx.doi.org/10.1016/j.scr.2019.101608DOI Listing

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