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| http://dx.doi.org/10.1021/acs.biochem.9b00688 | DOI Listing |
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Molecular Mechanisms of Heterosis and Its Applications in Tree Breeding: Progress and Perspectives.
Int J Mol Sci
November 2024
Key Laboratory of Eco-Environments of Three Gorges Reservoir Region, Ministry of Education, School of Life Sciences, Southwest University, Chongqing 400715, China.
Article Synopsis
- * The review highlights the genetic, molecular, and epigenetic mechanisms of heterosis, alongside modern predictive methods for improving tree species' growth rates and resistance to environmental stresses.
- * Future research directions emphasize the potential integration of advanced genomics, CRISPR gene-editing, and molecular marker-assisted selection to enhance tree hybrid breeding practices.
CRISPR-based genetic screens in human pluripotent stem cells derived neurons and brain organoids.
Cell Tissue Res
January 2025
Waisman Center, University of Wisconsin-Madison, Madison, WI, 53705, USA.
Recent large-scale genome-wide association and single-cell RNA sequencing (scRNA-seq) studies have uncovered disease-associated genetic risk factors and cell type-specific genetic alterations. However, our understanding of how these genetic variants cause diseases and the underlying mechanisms remains largely unknown. Functional genomics screens using CRISPR-based technologies offer an effective tool for studying genes relevant to disease phenotypes.
View Article and Find Full Text PDFAdenine base editors induce off-target structure variations in mouse embryos and primary human T cells.
Genome Biol
November 2024
Institute of Neuroscience, CAS Center for Excellence in Brain Science and Intelligence Technology, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai, 200031, China.
Background: The safety of CRISPR-based gene editing methods is of the utmost priority in clinical applications. Previous studies have reported that Cas9 cleavage induced frequent aneuploidy in primary human T cells, but whether cleavage-mediated editing of base editors would generate off-target structure variations remains unknown. Here, we investigate the potential off-target structural variations associated with CRISPR/Cas9, ABE, and CBE editing in mouse embryos and primary human T cells by whole-genome sequencing and single-cell RNA-seq analyses.
View Article and Find Full Text PDFEfficient CRISPR/Cas9 Knock-in Approaches for Manipulation of Endogenous Genes in Human B Lymphoma Cells.
Curr Protoc
November 2024
Division of Infectious Diseases, Department of Medicine, Brigham and Women's Hospital, Boston, Massachusetts.
Article Synopsis
- Traditional overexpression techniques struggle to accurately analyze protein-protein interactions and expression in B lymphocytes due to their resistance to lipid transfection.
- The article discusses advanced CRISPR/Cas9 knock-in methods that increase efficiency in tagging endogenous proteins, along with protocols for optimizing cutting efficiency and sgRNA selection.
- Detailed methodologies for engineering B lymphoma cells are provided, including assessing editing efficiency, designing repair templates, electroporation, and selecting engineered cells, enabling broader application in other cell types.
Multiplexed multimodal single-cell technologies: From observation to perturbation analysis.
Mol Cells
December 2024
Department of Biomedical Sciences, Yonsei University College of Medicine, Seoul, South Korea; Brain Korea 21 Project, Graduate School of Medical Science, Yonsei University College of Medicine, Seoul, South Korea. Electronic address:
Single-cell technologies have undergone a significant transformation, expanding from their initial focus on transcriptomics to encompass a diverse range of modalities. Recent advancements have markedly improved scalability and reduced costs, facilitating the processing of larger cell populations and broadening the scope of single-cell research. The incorporation of clustered regularly interspaced short palindromic repeats (CRISPR)-based perturbations has revolutionized the field by enabling precise functional genomics and detailed studies of gene regulation at the single-cell level.
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