Phage therapy is an abandoned antimicrobial therapy that has been resumed in recent years. In this study, we mutated a lysogenic phage from into a lytic phage (Ab105-2phiΔCI) that displayed antimicrobial activity against clinical strain Ab177_GEIH-2000 (isolated in the GEIH-REIPI Spanish Multicenter Study II 2000/2010, Umbrella Genbank Bioproject PRJNA422585, and for which meropenem and imipenem MICs of respectively, 32 µg/mL, and 16 µg/mL were obtained). We observed an in vitro synergistic antimicrobial effect (reduction of 4 log-7 log CFU/mL) between meropenem and the lytic phage in all combinations analyzed (Ab105-2phiΔCI mutant at 0.1, 1 and 10 MOI and meropenem at 1/4 and 1/8 MIC). Moreover, bacterial growth was reduced by 8 log CFU/mL for the combination of imipenem at 1/4 MIC plus lytic phage (Ab105-2phiΔCI mutant) and by 4 log CFU/mL for the combination of imipenem at 1/8 MIC plus lytic phage (Ab105-2phiΔCI mutant) at both MOI 1 and 10. These results were confirmed in an in vivo model (), and the combination of imipenem and mutant Ab105-2phiΔCI was most effective ( < 0.05). This approach could help to reduce the emergence of phage resistant bacteria and restore sensitivity to antibiotics used to combat multi-resistant strains of
Download full-text PDF |
Source |
---|---|
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6921023 | PMC |
http://dx.doi.org/10.3390/microorganisms7110556 | DOI Listing |
Sci Total Environ
December 2024
CSIRO Environment, Ecosciences Precinct, 41 Boggo Road, Dutton Park, QLD 4102, Australia.
This study investigated the potential role of phages in the dissemination of antimicrobial resistance genes (ARGs) and virulence factor genes (VFGs) in Escherichia coli (E. coli). A comprehensive in silico analysis of 18,410 phage sequences retrieved from the National Center for Biotechnology Information database (NCBI) revealed distinct carriage patterns for ARGs and VFGs between lytic, temperate, and chronic phage types.
View Article and Find Full Text PDFFront Microbiol
December 2024
Department of Biomedical Science, The Graduate School, Kyungpook National University, Daegu, Republic of Korea.
The emergence of the multidrug-resistant (MDR) ST131 clone has significantly impacted public health. With traditional antibiotics becoming less effective against MDR bacteria, there is an urgent need for alternative treatment options. This study aimed to isolate and characterize four lytic phages (EC.
View Article and Find Full Text PDFGeorgian Med News
October 2024
1G. Eliava Institute of Bacteriophage, Microbiology and Virology, Tbilisi, Georgia.
The emergence of antibiotic-resistant pathogens necessitates alternative therapies for treating microbial infections, especially in the oral cavity and upper respiratory tract. Our team has developed Phage Pastilles, a controlled-release formulation containing bacteriophages that target common pathogens, including Streptococcus pyogenes, Streptococcus salivarius, Staphylococcus aureus, Enterococcus faecalis, and E. coli.
View Article and Find Full Text PDFAMB Express
December 2024
Department of Biotechnology, Iranian Research Organization for Science and Technology (IROST), Tehran, Iran.
Antibiotics become less effective in treating infectious diseases as resistance increases. Staphylococcus aureus is a global problem due to its ability to form biofilms and resistance mechanisms. Phage endolysin is one of the most promising methods for combating antibiotic resistance.
View Article and Find Full Text PDFFront Microbiol
December 2024
Department of Food Science and Technology, The Ohio State University, Columbus, OH, United States.
Advances in bacteriophage genome sequencing and regulatory approvals of some bacteriophages in various applications have renewed interest in these antibacterial viruses as a potential solution to persistent food safety challenges. Here, we analyzed in depth the genome of the previously studied bacteriophage OSYSP (phage OSYSP), revealed its application-related characteristics, and optimized its enumeration techniques for facilitating industrial implementation. We previously sequenced phage OSYSP genome completely by combining results from Illumina Miseq and Ion Torrent sequencing platforms and completing the remaining sequence gaps using PCR.
View Article and Find Full Text PDFEnter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!