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Plant Growth-Promoting Bacteria (PGPB): Isolation and Screening of PGP Activities. | LitMetric

Plant Growth-Promoting Bacteria (PGPB): Isolation and Screening of PGP Activities.

Curr Protoc Plant Biol

Genetics Department, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Brazil.

Published: September 2017

AI Article Synopsis

  • * Utilizing biofertilizers and biocontrol strategies offers a more eco-friendly alternative to chemical methods in farming, leading to lower energy, environmental, and economic costs.
  • * The text outlines protocols for isolating potential beneficial bacteria from soil and plant roots and assessing their abilities to enhance plant growth through processes like nitrogen fixation and phosphate solubilization.

Article Abstract

Plant roots are associated with numerous and diverse types of beneficial and pathogenic microorganisms. Plant growth-promoting (rhizo)bacteria (PGPB or PGPR) are isolated from plants crops worldwide, and many of them are used as agricultural inoculants. Agricultural biofertilization and biocontrol of pathogens are eco-friendly alternatives to chemical usage and have less energy, environmental, and economic costs. PGPB isolation and evaluation are essentials steps for determining bacteria that are able to improve plant development and productivity. In this unit, we present protocols to isolate bacteria from soil and plant roots ("putative" diazotrophic and endospore-forming bacteria), as well to evaluate some of their beneficial characteristics for the promotion of plant growth (e.g., nitrogen fixation, production of indolic compounds and siderophores, phosphate solubilization, and 1-aminocyclopropane-1-carboxylate deaminase activity). © 2017 by John Wiley & Sons, Inc.

Download full-text PDF

Source
http://dx.doi.org/10.1002/pb.20054DOI Listing

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