AI Article Synopsis
- Recent advancements in metabolic engineering allow the use of engineered microbial strains to produce high-value chemicals, but challenges remain due to inefficiencies and metabolic strain within single organisms.
- To enhance product yields, researchers are exploring co-cultivation, where multiple engineered strains work together, distributing metabolic workloads and optimizing individual pathway components independently.
- This modular co-cultivation approach offers significant advantages for producing complex compounds, though it also presents challenges that need to be addressed to fully realize its potential in metabolic engineering.
Article Abstract
Recent advances in metabolic engineering enable the production of high-value chemicals via expressing complex biosynthetic pathways in a single microbial host. However, many engineered strains suffer from poor product yields due to redox imbalance and excess metabolic burden, and require compartmentalization of the pathway for optimal function. To address this problem, significant developments have been made towards co-cultivation of more than one engineered microbial strains to distribute metabolic burden between the co-cultivation partners and improve the product yield. In this emerging approach, metabolic pathway modules can be optimized separately in suitable hosts that will then be combined to enable optimal functionality of the complete pathway. This modular approach broadens the possibilities to fine tune sophisticated production platforms and thus achieve the biosynthesis of very complex compounds. Here, we review the different applications and the overall potential of natural and artificial co-cultivation systems in metabolic engineering in order to improve bioproduction/bioconversion. In addition to the several advantages over monocultures, major challenges and opportunities associated with co-cultivation are also discussed in this review.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6838517 | PMC |
| http://dx.doi.org/10.1016/j.mec.2019.e00095 | DOI Listing |
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