One of the most important resistant mechanisms in Gram-negative bacteria is extended spectrum β-lactamases (ESBLs). Harbour-related genes on plasmids, increase the risk of resistance transmission among commonly reported hospital infections. This study was designed to explore the dissemination of producing ESBLs on their plasmids recovered from the different wards of Amir-Al-Momenin burn center, Affiliated with Shiraz University of Medical Sciences. Among 256 isolates, 88 (34.38 %) strains were isolated from burn hospitalized patients. Samples were processed for antibiotic resistance using the Kirby-Bauer method while MIC was performed for colistin. MIC was used by the microdilution broth method as recommended by Clinical and Laboratory Standards Institute guidelines. Related studied genes were evaluated on extracted plasmids by the PCR method. According to the phenotypic and molecular steps, a total of 58 (65.91 %) and 74 (84.10 %) strains detected positive ESBLs, respectively. Based on antibiogram tests, a total of 63 (71.59 %) isolates were detected as multidrug resistant. All ESBL isolates showed identical antimicrobial susceptibility profiles. The genotypic prevalence of ESBLs for , , , and genes was 47.73, 78.41, 5.58, 3.41, 4.55 %, respectively. All strains producing ESBLs had plasmids containing related genes. The data indicated a high prevalence of ESBL among isolates in the southwest of the Iran burn center and their enzyme types were diverse.
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http://dx.doi.org/10.1099/jmm.0.001105 | DOI Listing |
Genome Med
December 2024
Laboratory of Medical Microbiology, Vaccine and Infectious Diseases Institute, University of Antwerp, Antwerp, Belgium.
Background: The impact of community carriage on the influx of extended-spectrum beta-lactamase-producing Enterobacterales (ESBL-E) into hospitals remains understudied. In this prospective 2-year single-centre study, we investigate the community ESBL-E influx and trace the colonisation, nosocomial acquisition, transmission, and infection dynamics of ESBL-producing Escherichia coli (ESBL-Ec) in non-ICU wards at a tertiary care hospital.
Methods: This study reports primary and post hoc outcomes of the clinical trial NCT01208519 in which hospitalised patients were screened for rectal carriage of ESBL-E.
Foods
December 2024
Centre for Applied Food Safety and Biotechnology, Department of Life Sciences, Central University of Technology, 1 Park Road, Bloemfontein 9300, South Africa.
Microb Pathog
December 2024
Laboratory of Research in Geo-Environment and Development of Spaces (LGEDE), Department of Biology, University of Mustapha Stambouli, BP 763, 29000, Mascara, Algeria.
Changes in antibiotic resistance of bacteria in three different wastewater treatment plants (WTP) were investigated to determine their role they play in the dissemination of multidrug resistant bacteria (MDR). Water samples were collected from downstream of three (WTP) from where pollution parameters were detected and high-performance Liquid chromatography (HPLC) coupled with an ultraviolet (UV) detector was used to measure antibiotic residues. In addition, 88 bacilli Gram-negative (BGN) were collected; activated sludge, aerated lagoons and natural lagoons, and tested for resistance against 18 βeta-lactam antibiotics.
View Article and Find Full Text PDFPharmacotherapy
November 2024
Anti-Infective Research Laboratory, Department of Pharmacy Practice, Eugene Applebaum College of Pharmacy and Health Sciences, Wayne State University, Detroit, Michigan, USA.
Antimicrobial resistance poses a significant public health challenge, particularly with the rise of gram-negative hospital-acquired infections resistant to carbapenems. Aztreonam-avibactam (ATM-AVI) is a promising new combination therapy designed to combat multidrug-resistant (MDR) gram-negative bacteria, including those producing metallo-β-lactamases (MBLs). Aztreonam, a monobactam antibiotic, is resistant to hydrolysis by MBLs but can be degraded by other β-lactamases.
View Article and Find Full Text PDFMicroorganisms
November 2024
Unidad Académica Ciencias de la Salud, Universidad Autónoma de Guadalajara, Zapopan 45129, Jalisco, Mexico.
Community-acquired urinary tract infections account for 15% of all outpatient use of antibiotics, and women are primarily affected; the major causative microorganism is uropathogenic (). Treatment is indicated for cystitis and pyelonephritis and includes B-lactams (amoxicillin-clavulanic acid or third-generation cephalosporins), fluoroquinolones (ciprofloxacin or levofloxacin), nitrofurantoin, fosfomycin, and trimethoprim-sulfamethoxazole. Resistance to antibiotic treatment is of concern; several mechanisms have been associated with the acquisition of genes that confer antimicrobial resistance to fluoroquinolones, which are often associated with other patterns of resistance, especially in extended-spectrum beta-lactamase (ESBL) producers.
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