An in-line holographic particle counter concept is presented and validated where multiple micrometer sized particles are detected in a three dimensional sampling volume, all at once. The proposed PIU is capable of detecting holograms of particles which sizes are in the lower μ m- range. The detection and counting principle is based on common image processing techniques using a customized HT with a result directly relating to the particle number concentration in the recorded sampling volume. The proposed counting unit is mounted ontop of a CNM for comparison with a commercial TSI-3775 CPC. The concept does not only allow for a precise in-situ determination of low particle number concentrations but also enables easy upscaling to higher particle densities (e.g., > 30 . 000 # c c m ) through its linear expandability and option of cascading. The impact of coincidence at higher particle densities is shown and two coincidence correction approaches are presented where, at last, its analogy to the coincidence correction methods used in state-of-the-art CPCs is identified.
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http://dx.doi.org/10.3390/s19224899 | DOI Listing |
Biosensors (Basel)
January 2025
Department of Electronic and Biomedical Engineering, Universitat de Barcelona, Martí i Franquès 1, 08028 Barcelona, Spain.
In recent years, the wine industry has been researching how to improve wine quality along the production value chain. In this scenario, we present here a new tool, MicroVi, a cost-effective chip-sized microscopy solution to detect and count yeast cells in wine samples. We demonstrate that this novel microscopy setup is able to measure the same type of samples as an optical microscopy system, but with smaller size equipment and with automated cell count configuration.
View Article and Find Full Text PDFJ Med Life
November 2024
Biophysics and Cellular Biotechnology Department, Carol Davila University of Medicine and Pharmacy, Bucharest, Romania.
Optical tweezers, which leverage the forces exerted by radiation pressure, have emerged as a pivotal technique for precisely manipulating and analyzing microscopic particles. Since Arthur Ashkin's ground-breaking work in the 1970s and the subsequent development of the single-beam optical trap in 1986, the capabilities of optical tweezers have expanded significantly, enabling the intricate manipulation of biological specimens at the micro- and nanoscale. This review elucidates the foundational principles of optical trapping and their extensive applications in the biomedical sciences.
View Article and Find Full Text PDFSci Rep
January 2025
Department of Electrical Engineering, Iran University of Science and Technology, Tehran, 16846-1314, Iran.
The holographic technique is one of the simplest methods for designing antennas based on metasurface. This paper presents a spoof surface plasmon polariton (SSPP) leaky-wave antenna (LWA) based on the concept of impedance modulated metasurfaces by the anisotropic holographic technique. Instead of parasitic elements, anisotropic SSPP elements are exploited to achieve radiation with circular polarization.
View Article and Find Full Text PDFEntropy (Basel)
December 2024
Department of Applied Mathematics, University of Washington, Seattle, WA 98195-3925, USA.
Statistical counting is the holographic observable to a statistical dynamics with finite states under independent and identically distributed sampling. Entropy provides the infinitesimal probability for an observed empirical frequency ν^ with respect to a probability prior p, when ν^≠p as N→∞. Following Callen's postulate and through Legendre-Fenchel transform, without help from mechanics, we show that an internal energy u emerges; it provides a linear representation of real-valued observables with full or partial information.
View Article and Find Full Text PDFPhys Rev Lett
November 2024
Higgs Centre for Theoretical Physics, University of Edinburgh, Edinburgh EH8 9YL, Scotland.
Thermal spectra of correlation functions in holographic 3D large-N conformal field theories (CFTs) correspond to quasinormal modes of classical gravity and other fields in asymptotically anti-de Sitter black hole spacetimes. Using general properties of such spectra along with constraints imposed by the S duality (or the particle-vortex duality), we derive a spectral duality relation that all such spectra must obey. Its form is universal and relates infinite products over QNMs with bulk algebraically special frequencies.
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