Prevention of damage caused by corona discharge-generated reactive oxygen species under electrostatic aerosol-to-hydrosol sampling.

Human exposure to airborne pathogens is a major cause of health concerns; therefore, it is imperative to monitor, sample, and detect airborne bio-particles. Among various bio-aerosol sampling methods, electrostatic precipitation (EP) is an efficient technique for capturing bio-aerosols as hydrosols due to a lower pressure drop and less damage to sensitive bio-particles. Corona discharge is the main EP mechanism; however, this inevitably generates reactive oxygen species (ROS), which can be transported and dissolved in the sampling liquid. ROS can modify cellular component structures and damage DNA. Additionally, during the sampling process, the liquid flow rate and sampling liquid type can highly affect sampling efficiency. Here, different liquid types and flow rates are examined and ascorbic acid (AA), known as vitamin C, is added to prevent bio-particle damage. However, a high AA concentration can cause oxidative damage. Therefore, the optimal AA concentration should be chosen to obtain the greatest protective effect.