Objectives: This study aimed to assess the effects of L-carnitine on oxidative stress in human erythrocytes during storage.
Background: Using antioxidants as components of blood storage solutions may combat the effects of storage-induced oxidative stress on erythrocytes.
Methods: Blood from male adults was stored at 4 °C for 55 days in citrate phosphate dextrose adenine solution, without L-carnitine (Control) and with L-carnitine as an additive (at concentrations of 10, 30 and 60 mM - Experiments). Every fifth day, erythrocyte markers (morphology, count, haemoglobin, haemolysis and osmotic fragility), antioxidant defences (antioxidant enzymes and total antioxidant capacity) and oxidative stress markers (superoxides, lipid peroxidation and protein oxidation products) were analysed.
Results: Oxidative damage was observed in controls (day 25 onwards) and in experiments (day 35 onwards). L-carnitine (10 and 30 mM) protected erythrocytes from damage up to day 35 by maintaining haemoglobin and lipid peroxidation, assisting antioxidant enzymes and increasing antioxidant capacity by elevating sulfhydryls and ascorbic acid. L-carnitine was beneficial in prolonging storage up to 55 days but could not prevent oxidative damage completely in terms of haemolysis and osmotic fragility.
Conclusions: L-carnitine ameliorated oxidative stress, but combinations with other antioxidants may provide comprehensive protection to erythrocytes during storage.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1111/tme.12645 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Exploring the role of oxidative stress in carotid atherosclerosis: insights from transcriptomic data and single-cell sequencing combined with machine learning.
Biol Direct
January 2025
National Key Laboratory for Innovation and Transformation of Luobing Theory; The Key Laboratory of Cardiovascular Remodeling and Function Research, Chinese Ministry of Education, Chinese National Health Commission and Chinese Academy of Medical Sciences, Jinan, China.
Background: Carotid atherosclerotic plaque is the primary cause of cardiovascular and cerebrovascular diseases. It is closely related to oxidative stress and immune inflammation. This bioinformatic study was conducted to identify key oxidative stress-related genes and key immune cell infiltration involved in the formation, progression, and stabilization of plaques and investigate the relationship between them.
View Article and Find Full Text PDFFrom micro to macro, nanotechnology demystifies acute pancreatitis: a new generation of treatment options emerges.
J Nanobiotechnology
January 2025
Department of Gastroenterology, Shanghai Institute of Pancreatic Diseases, National Key Laboratory of Immunity and Inflammation, Changhai Hospital, Naval Medical University, Shanghai, 200433, China.
Acute pancreatitis (AP) is a disease characterized by an acute inflammatory response in the pancreas. This is caused by the abnormal activation of pancreatic enzymes by a variety of etiologic factors, which results in a localized inflammatory response. The symptoms of this disease include abdominal pain, nausea and vomiting and fever.
View Article and Find Full Text PDFHarnessing multivariate insights coupled with susceptibility indices to reveal morpho-physiological and biochemical traits in heat tolerance of cotton.
BMC Plant Biol
January 2025
Plant Breeding and Genetics Division, Nuclear Institute for Agriculture and Biology (NIAB), Faisalabad, Pakistan.
Cotton is essential for the global textile industry however, climate change, especially extreme temperatures, threatens sustainable cotton production. This research aims to identify breeding strategies to improve heat tolerance and utilize stress-resistant traits in cotton cultivars. This study investigated heat tolerance for 50 cotton genotypes at the seedling stage by examining various traits at three temperatures (32 °C, 45 °C and 48 °C) in a randomized plot experiment.
View Article and Find Full Text PDFThe prospective therapeutic benefits of sesamol: neuroprotection in neurological diseases.
Nutr Neurosci
January 2025
State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macau, People's Republic of China.
Oxidative stress is recognized as a critical contributor to the advancement of neurological diseases, thereby rendering the alleviation of oxidative stress a pivotal strategy in the therapeutic management of such conditions. Sesamol, the principal constituent of sesame oil, has been the subject of extensive research due to its significant antioxidant properties, especially its ability to effectively counteract oxidative stress within the central nervous system and confer neuroprotection. While sesamol demonstrates potential in the treatment and prevention of neurological diseases, its modulation of oxidative stress is complex and not yet fully understood.
View Article and Find Full Text PDFCadmium Pollution Deteriorates the Muscle Quality of Labeo rohita by Altering Its Nutrients and Intestinal Microbiota Diversity.
Biol Trace Elem Res
January 2025
Yunnan Collaborative Innovation Center for Plateau Lake Ecology and Environmental Health, College of Agronomy and Life Sciences, Kunming University, Kunming, 650214, China.
The detrimental effects of cadmium (Cd), a hazardous heavy metal, on fish have triggered global concerns. While the ecotoxicity of Cd on fish has been investigated, the impact of Cd on muscle quality and its correlation with the gut microbiota in fish remains scarce. To comprehensively uncover Cd effects based on preliminary muscle Cd deposition, relevant studies, and ecological Cd pollution data, we exposed Labeo rohita to Cd under concentrations of 0.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!