AI Article Synopsis
- A DNA-based nanosensor was created to detect specific genes in milk samples, utilizing a specialized single-stranded DNA probe attached to a composite electrode made of carbon nanotubes and gold nanoparticles.
- The fabrication process involved EDC-cross-linking chemistry, and the sensor's performance was evaluated through electrochemical techniques like cyclic voltammetry and differential pulse voltammetry, with impressive sensitivity and a very low detection limit.
- Validation tests showed the nanosensor's stability and sensitivity for up to six months, maintaining accuracy even when compared against traditional detection methods.
Article Abstract
In this study, a DNA-based nanosensor using specific NH labeled single standard probe was developed against gene of in milk samples. The single-stranded DNA probe was immobilized on carboxylated multiwalled carbon nanotube and gold nanoparticle (c-MWCNT/AuNP) electrode using 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC): -hydroxy succinimide-based cross-linking chemistry. Electrochemical characterization was performed using cyclic voltammetry (CV) and Differential Pulse Voltammetry (DPV) techniques. The electrode surface at each step of fabrication was characterized using scanning electron microscopy. The sensitivity and lower limit of detection were found to be 728.42 (μA/cm)/ng and 1.8 pg/6 μl (0.3 pg/ml), respectively, with regression coefficient ( ) of 0.843 using DPV. The sensor was further validated using raw and artificial milk samples, and results were compared with conventional methods of detection. The developed sensor was found to be highly sensitive and stable up to 6 months, with only 10% loss of initial peak current in CV analysis on storage at 4 °C.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6816711 | PMC |
| http://dx.doi.org/10.1007/s13205-019-1957-4 | DOI Listing |
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