AI Article Synopsis
- Two bacterial isolates from the Barents Sea produced extracts with antibacterial properties, leading to the discovery of a lipopeptide named Lipid 430.
- Mass spectrometry and nuclear resonance spectroscopy helped identify Lipid 430's structure and demonstrated its antibacterial activity, as well as a cytotoxic effect on the A2058 human melanoma cell line.
- The study revealed that Lipid 430's mechanism against melanoma cells is more complex than just breaking down cell membranes, as it did not cause immediate cell lysis.
Article Abstract
Two bacterial isolates from the Barents Sea, both belonging to the genus , were found to yield extracts with anti-bacterial bioactivity. Mass spectrometry guided dereplication and purification of the active extracts lead to the isolation of the same active principle in both extracts. The structure of the bioactive compound was identified via mass spectrometry and nuclear resonance spectroscopy and it turned out to be the known lipopeptide Lipid 430. We discovered and determined its previously unknown anti-bacterial activity against and revealed a cytotoxic effect against the A2058 human melanoma cell line at significantly lower concentrations compared to its anti-bacterial concentration. Flow cytometry and microscopy investigations of the cytotoxicity against the melanoma cell line indicated that Lipid 430 did not cause immediate cell lysis. The experiments with melanoma cells suggest that the compound functions trough more complex pathways than acting as a simple detergent.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6864645 | PMC |
| http://dx.doi.org/10.3390/molecules24213991 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!