AI Article Synopsis
- Lung cancer is a major health issue globally, and this study aims to explore mechanisms behind non-small cell lung cancer to discover new treatment options.
- The researchers used various methods, including RT-PCR and Western blot, to establish a connection between the transcription factor E2F1 and the IRF5 gene in both normal and cancerous lung tissues.
- Findings indicate that E2F1 positively influences the expression of IRF5, suggesting its crucial role in activating IRF5 transcription in non-small cell lung cancer.
Article Abstract
Purpose: Lung cancer is the most common malignant tumor in the world, and its incidence and mortality are very high. This study focuses on the mechanism of non-small cell lung cancer to find new therapeutic targets.
Methods: We used RT-PCR and Western blot to verify the linear relationship between E2F1 and IRF5 in normal lung tissue and lung cancer tissues. Secondly, we used overexpression and knock down E2F1 in cell lines to detect the expression of IRF5. The prime enzyme reporter plasmid verified that E2F1 binds to the core promoter region of IRF5; finally, CHIP experiments demonstrated that E2F1 binds directly to IRF5.
Results: We verified that E2F1 and IRF5 are decreased in patient tissues, and there is a strong linear relationship between E2F1 and IRF5. Secondly, we used overexpression of E2F1 or E2F1 siRNA transfected into HCC827 cells and found that E2F1 positively regulates the activity of the IRF5 promoter and the mRNA level of IRF5. Finally, the results of a chromatin immunoprecipitation assay demonstrated that E2F1 bound to the promoter region of IRF5 in vitro. These results suggested that the E2F1 transcription factor is the primary determinant for activating the basal transcription of the IRF5.
Conclusion: The transcription factor E2F1 positively regulates IRF5 in non-small cell lung cancer.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6711570 | PMC |
| http://dx.doi.org/10.2147/OTT.S215701 | DOI Listing |
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