Background: Seed germination, the foundation of plant propagation, involves a series of changes at the molecular level. Poplar is a model woody plant, but the molecular events occurring during seed germination in this species are unclear.
Results: In this study, we investigated changes in gene transcriptional levels during different germination periods in poplar by high-throughput sequencing technology. Analysis of genes expressed at specific germination stages indicated that these genes are distributed in many metabolic pathways. Enrichment analysis of significantly differentially expressed genes based on hypergeometric testing revealed that multiple pathways, such as pathways related to glycolysis, lipid, amino acid, protein and ATP synthesis metabolism, changed significantly at the transcriptional level during seed germination. A comparison of ΣZ values uncovered a series of transcriptional changes in biological processes related to primary metabolism during poplar seed germination. Among these changes, genes related to CHO metabolism were the first to be activated, with subsequent expression of genes involved in lipid metabolism and then those associated with protein metabolism. The pattern of metabolomic and physiological index changes further verified the sequence of some biological events.
Conclusions: Our study revealed molecular events occurring at the transcriptional level during seed germination and determined their order. These events were further verified by patterns of changes of metabolites and physiological indexes. Our findings lay a foundation for the elucidation of the molecular mechanisms responsible for poplar seed germination.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6829952 | PMC |
| http://dx.doi.org/10.1186/s12864-019-6180-5 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Comparative pericarp biomechanics and germination physiology of Raphanus raphanistrum and Raphanus pugioniformis indehicent fruits.
Ann Bot
January 2025
Seed Biology and Technology Group, Department of Biological Sciences, Royal Holloway University of London, TW20 0EX, Egham, United Kingdom.
The biomechanical, morphological and ecophysiological properties of plant seed/fruit structures are adaptations that support survival in unpredictable environments. High phenotypic variability of noxious and invasive weed species such as Raphanus raphanistrum (wild radish) allow diversification into new environmental niches. Dry indehiscent fruits (thick and lignified pericarp [fruit coat] enclosing seeds) have evolved many times independently.
View Article and Find Full Text PDFMethodological study on coal-based microbial modification of mineral black clay to overcome plant growth challenges on open-pit mine dumps in cold regions.
MethodsX
June 2025
CUMT-UCASAL Joint Research Center for Biomining and Soil Ecological Restoration, State Key Laboratory for Fine Exploration and Intelligent Development of Coal Resources, China University of Mining and Technology, Xuzhou, Jiangsu province, 221116, PR China.
A critical challenge in ecological restoration of open-pit mine dumps in cold regions with limited topsoil resources is how to rapidly mitigate the plant growth-inhibitory effects of mineral black clay, thereby converting it into arable soil. Leveraging the high degradation capacity of coal seam-associated microorganisms on fossil carbon materials, combined with soil conditioning techniques, this study developed a microbial-based approach for modifying black clay. Seed germination experiments informed both laboratory and field trial designs.
View Article and Find Full Text PDFEpitranscriptome profiles reveal participation of the RNA methyltransferase gene OsMTA1 in rice seed germination and salt stress response.
BMC Plant Biol
January 2025
State Key Laboratory of Crop Gene Resources and Breeding/Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, South Zhong-Guan-Cun Street 12#, Beijing, 100081, China.
Background: RNA m6A methylation installed by RNA methyltransferases plays a crucial role in regulating plant growth and development and environmental stress responses. However, the underlying molecular mechanisms of m6A methylation involved in seed germination and stress responses are largely unknown. In the present study, we surveyed global m6A methylation in rice seed germination under salt stress and the control (no stress) using an osmta1 mutant and its wild type.
View Article and Find Full Text PDFAnthocyanin can improve the survival of rice seeds from solar light outside the international space station.
Life Sci Space Res (Amst)
February 2025
School of Life Sciences, Tokyo University of Pharmacy and Life Sciences, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392, Japan.
A purple-pigmented (purple) rice seeds containing an anthocyanin, a major class of flavonoids, and their isogenic non-pigmented (white) seeds were exposed outside of the international space station (ISS) to evaluate the impact of anthocyanin on seed viability in space. The rice seeds were placed in sample plates at the exposed facility of ISS for 440 days, with the bottom layer seeds exposed to space radiation and the top layer seeds exposed to both solar light and space radiation. Though the seed weight of both purple and white seeds decreased after exposure to outer space, growth percentages after germination of purple and white seeds in the top layer were 55 and 15 %, respectively, compared to those in the bottom layer 100 and 70 %, respectively.
View Article and Find Full Text PDFOverexpressing OsNF-YB12 elevated the content of jasmonic acid and impaired drought tolerance in rice.
Plant Sci
January 2025
Shanghai Agrobiological Gene Center, Shanghai, 201106 China; Key Laboratory of Grain Crop Genetic Resources Evaluation and Utilization, Ministry of Agriculture and Rural Affairs, Shanghai, 201106, China. Electronic address:
Nuclear factor Y (NF-Y) is an evolutionarily conserved heterotrimeric transcription factor in eukaryotes. In a previous study, OsNF-YB12 was confirmed to be associated with drought tolerance using the Ecotilling method. In this study, real-time quantitative RT-PCR revealed that OsNF-YB12 was induced by various abiotic stresses and phytohormones, with expression levels differing between leaves and roots.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!