A smartphone-integrated ratiometric fluorescent sensing system (DPA-Ce-GMP-Eu) for visual and point-of-care testing (POCT) of tetracycline with high sensitivity and accuracy was developed. The blue fluorescence of DPA-Ce-GMP was changed into red by doping with Eu duo to the energy transfer from Ce to Eu. Upon exposure to tetracycline, coordination between Eu and tetracycline blocks energy transfer from Ce to Eu, converting the fluorescent color from red to blue. The tetracycline detection can be realized within a wide concentration range from 0.01 μM to 45 μM. The limit of detection (LOD) reaches as low as 6.6 nM. To realize quantitative point-of-care detection in real samples, a portable device with smartphone as signal reader and analyzer is further designed to integrate with the DPA-Ce-GMP-Eu sensing platform. The Color Picker APP installed in the smartphone can convert the Red, Green and Blue (RGB) channels of the fluorescence images into digital values. With milk as real sample, tetracycline can be on-site detected with LOD of 10.8 nM. This developed platform presents a great promise for POCT in practical application with merits of low cost, easy carry, simple operation, and excellent selectivity and repeatability.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.bios.2019.111791 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
A smartphone-integrated bimetallic ratiometric fluorescent probe for specific visual detection of tetracycline antibiotics in food samples and latent fingerprinting.
Food Chem
February 2025
School of Chemistry and Chemical Engineering, Lanzhou Jiaotong University, Lanzhou, Gansu 730070, PR China.
Designing and preparing highly sensitive and accurate fluorescent chemosensors for monitoring tetracycline antibiotics remains a challenge. Herein, a fluorescent chemosensor based on lanthanide metal-organic frameworks (Ln-MOFs) is proposed to realize high-precision monitoring by adjusting the ratio of lanthanide ions. Ln-MOFs with good aqueous stability were prepared by a solvothermal method using Eu, Tb and the ligand 4,4',4″-s-triazine-2,4,6-triyltribenzoic acid (HTATB) in DMF/NMP/HO.
View Article and Find Full Text PDFA handheld fluorescent platform integrated with a Sm(III)-CdTe quantum dot-based ratiometric nanoprobe for point-of-use determination of phosphate.
Nanoscale
November 2024
College of Civil and Transportation Engineering, Shenzhen University, Shenzhen, Guangdong 518060, P.R. China.
Article Synopsis
- Phosphate (Pi) is essential for physiological processes and environmental health, prompting the need for a simple sensor to quickly detect it for health and conservation purposes.
- This study presents a dual-emission ratiometric fluorescence sensor that uses samarium ions and cadmium telluride quantum dots for sensitive Pi detection, indicated by a color change from purple to blue due to fluorescence recovery.
- The developed portable lab-on-paper device allows for quick Pi detection using a smartphone, showing effective performance in environmental and biological samples, which highlights a new approach for affordable and precise target detection.
FRET-Based Dual-Color Carbon Dot Ratiometric Fluorescent Sensor Enables the Smartphone-Integrated Device for Noninvasive and Portable Diagnosis of Chronic Kidney Disease.
Anal Chem
November 2024
Department of Pathology, Cancer Hospital of Zhejiang Province, Hangzhou Institute of Medicine, Chinese Academy of Sciences, Hangzhou, Zhejiang 310022, P. R. China.
Cystatin C (Cys C), a crucial renal disease marker for chronic kidney disease (CKD), plays a vital role in early diagnosis and treatment guidance. However, most current methods for detecting Cys C rely on a single signal and find it difficult to perform noninvasive and portable diagnosis. Here, we developed a ratiometric fluorescent carbon dot (CD) detection system for point-of-care testing (POCT) of Cys C through fluorescence resonance energy transfer (FRET).
View Article and Find Full Text PDFA smartphone-integrated ratiometric fluorescent sensor for ascorbic acid determination using microplasma-enabled carbon dots and rhodamine B.
Food Chem
January 2025
College of Chemistry and Material Science, Sichuan Normal University, Chengdu, Sichuan 610068, China. Electronic address:
Article Synopsis
- A smartphone-assisted sensing platform using nitrogen-doped carbon dots and Rhodamine B was developed to measure ascorbic acid (AA) levels in fruits through a fluorescence quenching method.
- The nitrogen-doped carbon dots, which emit blue light, were created using a microplasma technique and were found to have a quantum yield of 18%.
- The system allows for both visual and quantitative measurements, achieving detection limits of 0.98 μM and 2.90 μM using fluorescence spectroscopy and a smartphone, respectively.
A Smartphone-Integrated Ratiometric Fluorescence Sensor for the Ultrasensitive and Selective Detection of 5-Heneicosylresorcinol in Whole Wheat Foods.
J Agric Food Chem
September 2024
Key Laboratory of Geriatric Nutrition and Health, Ministry of Education, Beijing Technology and Business University, 11 Fucheng Road, Beijing 100048, China.
Precise on-site monitoring of alkylresorcinols, a vital biomarker, is crucial for verifying whole wheat foods and accurately quantifying the whole wheat content in various consumer and industrial products. Herein, for the first time, we introduce a novel ratiometric fluorescence sensor (CDs@ZIF-8/CdTe@MIP) for ultrasensitive and selective detection of alkylresorcinols. 5-Heneicosylresorcinol (C21:0 AR), the primary alkylresorcinol homologue in whole wheat grains, was selected as the target analyte.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!