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2045-2322912019Oct31Scientific reportsSci RepSurface modification of polyester fabric using plasma-dendrimer for robust immobilization of glucose oxidase enzyme.15730157301573010.1038/s41598-019-52087-8Robust immobilization of glucose oxidase (GOx) enzyme was achieved on poly(ethylene terephthalate) nonwoven fabric (PN) after integration of favourable surface functional groups through plasma treatments [atmospheric pressure-AP or cold remote plasma-CRP (N2 + O2)] and/or chemical grafting of hyperbranched dendrimers [poly-(ethylene glycol)-OH or poly-(amidoamine)]. Absorption, stability, catalytic behavior of immobilized enzymes and reusability of resultant fibrous bio-catalysts were comparatively studied. Full characterization of PN before and after respective modifications was carried out by various analytical, instrumental and arithmetic techniques. Results showed that modified polyester having amine terminal functional groups pledged better surface property providing up to 31% enzyme loading, and 81% active immobilized enzymes. The activity of the enzyme was measured in terms of interaction aptitude of GOx in a given time to produce hydrogen peroxide using colorimetric assay. The immobilized GOx retained 50% of its original activity after being reused six (06) times and exhibited improved stability compared with the free enzyme in relation to temperature. The reaction kinetics, loading efficiency, leaching, and reusability analysis of enzyme allowed drawing a parallel to the type of organic moiety integrated during GOx immobilization. In addition, resultant fibrous bio-catalysts showed substantial antibacterial activity against pathogenic bacteria strains (Staphylococcus epidermidis and Escherichia coli) in the presence of oxygen and glucose. These results are of great importance because they provide proof-of-concept for robust immobilization of enzymes on surface-modified fibrous polyester fabric for potential bio-industrial applications.MorshedMohammad NeazMNTextile Material Technology, Department of Textile Technology, The Swedish School of Textiles, Faculty of Textiles, Engineering and Business, University of Borås, SE-50190, Borås, Sweden. mohammad_neaz.morshed@hb.se.Ecole Nationale Supérieure des Arts et Industries Textiles (ENSAIT), GEMTEX Laboratory, 2 allée Louise et Victor Champier BP 30329, 59056, Roubaix, France. mohammad_neaz.morshed@hb.se.Université de Lille, Nord de France, F-59000, Lille, France. mohammad_neaz.morshed@hb.se.College of Textile and Clothing Engineering, Soochow University, 215006, Suzhou, China. mohammad_neaz.morshed@hb.se.BeharyNemeshwareeNEcole Nationale Supérieure des Arts et Industries Textiles (ENSAIT), GEMTEX Laboratory, 2 allée Louise et Victor Champier BP 30329, 59056, Roubaix, France.Université de Lille, Nord de France, F-59000, Lille, France.BouaziziNabilNEcole Nationale Supérieure des Arts et Industries Textiles (ENSAIT), GEMTEX Laboratory, 2 allée Louise et Victor Champier BP 30329, 59056, Roubaix, France.Université de Lille, Nord de France, F-59000, Lille, France.GuanJinpingJCollege of Textile and Clothing Engineering, Soochow University, 215006, Suzhou, China.ChenGuoqiangGCollege of Textile and Clothing Engineering, Soochow University, 215006, Suzhou, China.NierstraszVincentVTextile Material Technology, Department of Textile Technology, The Swedish School of Textiles, Faculty of Textiles, Engineering and Business, University of Borås, SE-50190, Borås, Sweden.engJournal ArticleResearch Support, Non-U.S. Gov't20191031
EnglandSci Rep1015632882045-23220Dendrimers0Enzymes, Immobilized0Plasma Gases0PolyestersBBX060AN9VHydrogen PeroxideEC 1.1.3.4Glucose OxidaseIMBiocatalysisColorimetryDendrimerschemistryEnzyme StabilityEnzymes, ImmobilizedchemistrymetabolismpharmacologyEscherichia colidrug effectsGlucose OxidasechemistrymetabolismHydrogen PeroxidechemistrymetabolismKineticsPlasma GaseschemistryPolyesterschemistryStaphylococcus epidermidisdrug effectsThe authors declare no competing interests.
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3151501MCID_676f0862d0c781be9a0cbbf7 39708873 39708778 39703712 39700475 39696903 robust "robust"[All Fields] OR "robustness"[All Fields] immobilization "immobile"[All Fields] OR "immobilisation"[All Fields] OR "immobilization"[MeSH Terms] OR "immobilization"[All Fields] OR "immobilise"[All Fields] OR "immobilised"[All Fields] OR "immobiliser"[All Fields] OR "immobilises"[All Fields] OR "immobilising"[All Fields] OR "immobilisations"[All Fields] OR "immobilize"[All Fields] OR "immobilizations"[All Fields] OR "immobilized"[All Fields] OR "immobilizer"[All Fields] OR "immobilizers"[All Fields] OR "immobilizes"[All Fields] OR "immobilizing"[All Fields] ("robust"[All Fields] OR "robustness"[All Fields]) AND ("immobile"[All Fields] OR "immobilisation"[All Fields] OR "immobilization"[MeSH Terms] OR "immobilization"[All Fields] OR "immobilise"[All Fields] OR "immobilised"[All Fields] OR "immobiliser"[All Fields] OR "immobilises"[All Fields] OR "immobilising"[All Fields] OR "immobilisations"[All Fields] OR "immobilize"[All Fields] OR "immobilizations"[All Fields] OR "immobilized"[All Fields] OR "immobilizer"[All Fields] OR "immobilizers"[All Fields] OR "immobilizes"[All Fields] OR "immobilizing"[All Fields]) trying2...
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Surface modification of polyester fabric using plasma-dendrimer for robust immobilization of glucose oxidase enzyme. | LitMetric

Surface modification of polyester fabric using plasma-dendrimer for robust immobilization of glucose oxidase enzyme.

Sci Rep

Textile Material Technology, Department of Textile Technology, The Swedish School of Textiles, Faculty of Textiles, Engineering and Business, University of Borås, SE-50190, Borås, Sweden.

Published: October 2019

Robust immobilization of glucose oxidase (GOx) enzyme was achieved on poly(ethylene terephthalate) nonwoven fabric (PN) after integration of favourable surface functional groups through plasma treatments [atmospheric pressure-AP or cold remote plasma-CRP (N + O)] and/or chemical grafting of hyperbranched dendrimers [poly-(ethylene glycol)-OH or poly-(amidoamine)]. Absorption, stability, catalytic behavior of immobilized enzymes and reusability of resultant fibrous bio-catalysts were comparatively studied. Full characterization of PN before and after respective modifications was carried out by various analytical, instrumental and arithmetic techniques. Results showed that modified polyester having amine terminal functional groups pledged better surface property providing up to 31% enzyme loading, and 81% active immobilized enzymes. The activity of the enzyme was measured in terms of interaction aptitude of GOx in a given time to produce hydrogen peroxide using colorimetric assay. The immobilized GOx retained 50% of its original activity after being reused six (06) times and exhibited improved stability compared with the free enzyme in relation to temperature. The reaction kinetics, loading efficiency, leaching, and reusability analysis of enzyme allowed drawing a parallel to the type of organic moiety integrated during GOx immobilization. In addition, resultant fibrous bio-catalysts showed substantial antibacterial activity against pathogenic bacteria strains (Staphylococcus epidermidis and Escherichia coli) in the presence of oxygen and glucose. These results are of great importance because they provide proof-of-concept for robust immobilization of enzymes on surface-modified fibrous polyester fabric for potential bio-industrial applications.

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