Integrating cytometric analysis of cells, mitochondria, and other polynucleotide-containing biological particles with high-throughput single particle sequencing would provide an ultimate bioanalytical tool, simultaneously assessing phenotype, functionality, genome, and transcriptome of each particle in a large population. Here, we describe how such integration could be performed by adapting existing, well-established technologies.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.tibtech.2019.09.002 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Immunogenic dying cells elicit potent anti-tumor T cell immunity against lung metastasis and tumorigenesis.
J Cancer Res Clin Oncol
January 2025
Medical Research Center, Binzhou Medical University Hospital, Binzhou, Shandong, 256600, P.R. China.
Purpose: Immune checkpoint blockades (ICBs) are promising, however they do not fit all types of tumor, such as those lack of tumor antigens. Induction of potent anti-tumor T cell immunity is critical for cancer therapy. In this study, we investigated the efficacy of immunotherapy via the immunogenic cell death (ICD) dying tumor cells in mouse models of lung metastasis and tumorigenesis.
View Article and Find Full Text PDFMicrofluidic mixing probe: generating multiple concentration-varying flow dipoles.
Sci Rep
January 2025
Division of Engineering, New York University Abu Dhabi, Abu Dhabi, United Arab Emirates.
This study advances microfluidic probe (MFP) technology through the development of a 3D-printed Microfluidic Mixing Probe (MMP), which integrates a built-in pre-mixer network of channels and features a lined array of paired injection and aspiration apertures. By combining the concepts of hydrodynamic flow confinements (HFCs) and "Christmas-tree" concentration gradient generation, the MMP can produce multiple concentration-varying flow dipoles, ranging from 0 to 100%, within an open microfluidic environment. This innovation overcomes previous limitations of MFPs, which only produced homogeneous bioreagents, by utilizing the pre-mixer to create distinct concentration of injected biochemicals.
View Article and Find Full Text PDFComprehensive benefits evaluation of low impact development using scenario analysis and fuzzy decision approach.
Sci Rep
January 2025
Business School, Sichuan University, 610059, Chengdu, China.
The comprehensive benefit evaluation of LID based on multi-criteria decision-making methods faces technical issues such as the uncertainties and vagueness in hybrid information sources, which can affect the overall evaluation results and ranking of alternatives. This study introduces a multi-indicator fuzzy comprehensive benefit evaluation approach for the selection of LID measures, aiming to provide a robust and holistic framework for evaluating their benefits at the community level. The proposed methodology integrates quantitative environmental and economic indicators with qualitative social benefit indicators, combining the use of the Storm Water Management Model (SWMM) and ArcGIS for scenario-based analysis, and the use of hesitant fuzzy language sets and Technique for Order of Preference by Similarity to Ideal Solution (TOPSIS) for decision-making.
View Article and Find Full Text PDFFrequency-specific changes in prefrontal activity associated with maladaptive belief updating in volatile environments in euthymic bipolar disorder.
Transl Psychiatry
January 2025
Department of Psychology, Goldsmiths University of London, London, UK.
Bipolar disorder (BD) involves altered reward processing and decision-making, with inconsistencies across studies. Here, we integrated hierarchical Bayesian modelling with magnetoencephalography (MEG) to characterise maladaptive belief updating in this condition. First, we determined if previously reported increased learning rates in BD stem from a heightened expectation of environmental changes.
View Article and Find Full Text PDFQuantitative pre-clinical imaging of hypoxia and vascularity using MRI and PET.
Methods Cell Biol
January 2025
Translational Radiomics, Luxembourg Institute of Health, Luxembourg City, Luxembourg; In-Vivo Imaging Platform, Luxembourg Institute of Health, Luxembourg City, Luxembourg.
During hypoxia, tissues are subjected to an inadequate oxygen supply, disrupting the balance needed to maintain normal function. This deficiency can occur due to reduced oxygen delivery caused by impaired blood flow or a decline in the blood's ability to carry oxygen. In tumors, hypoxia and vascularization play crucial roles, shaping their microenvironments and influencing cancer progression, response to treatment and metastatic potential.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!