Leukotoxic potential of strains has been studied by the use of several methods, and results differ depending on the methods used. The aim of the present study was to perform a comprehensive examination of the leukotoxic potential of a collection of strains by use of three quantitative methods, Western blotting, ELISA, and mRNA expression assay and compare these results with previous data obtained by a cell lysis assay. A higher leukotoxic potential among JP2 genotype strains compared to non-JP2 genotype strains of was found by Western blotting, ELISA and mRNA expression assay. Leukotoxicity as determined by cell lysis assay showed a variation among strains examined, not only depending on being part of JP2 genotype . non-JP2 genotype group of . The leukotoxicity of strains as determined by cell lysis assay did not correspond to the leukotoxic potential of strains as determined by three quantitative methods. A comparison of the results obtained by ELISA and mRNA expression assay showed a reasonable correlation between these two methods. It seems important to use more than one method to assess the LtxA-related virulence capacity of in order to obtain comprehensive understanding of the leukotoxic potential of strains.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6963569 | PMC |
| http://dx.doi.org/10.3390/pathogens8040211 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Occurrence of and Its JP2 Genotype in a Cohort of 220 Western Australians with Unstable Periodontitis.
Microorganisms
November 2024
Dental School, The University of Western Australia, Nedlands, WA 6009, Australia.
Article Synopsis
- The study aimed to investigate the presence of a specific pathogen and its JP2 genotype in 200 Western Australians with periodontitis.
- A total of 220 patients provided various oral samples for analysis, which showed a 28.18% prevalence of the pathogen, with higher rates in unstimulated saliva compared to cheek swabs and subgingival plaque.
- Key risk factors for severe periodontitis included younger age, positive family history of periodontal disease, poor oral hygiene, and irregular dental visits, while no JP2 genotype was detected in the cohort.
Improved prediction of radiation pneumonitis by combining biological and radiobiological parameters using a data-driven Bayesian network analysis.
Transl Oncol
July 2022
Department of Radiation Oncology, Medical School, The University of Michigan Medical School, University of Michigan, Ann Arbor, MI 48109-2026, USA. Electronic address:
Grade 2 and higher radiation pneumonitis (RP2) is a potentially fatal toxicity that limits efficacy of radiation therapy (RT). We wished to identify a combined biomarker signature of circulating miRNAs and cytokines which, along with radiobiological and clinical parameters, may better predict a targetable RP2 pathway. In a prospective clinical trial of response-adapted RT for patients (n = 39) with locally advanced non-small cell lung cancer, we analyzed patients' plasma, collected pre- and during RT, for microRNAs (miRNAs) and cytokines using array and multiplex enzyme linked immunosorbent assay (ELISA), respectively.
View Article and Find Full Text PDFMultilocus Sequence Typing of Non-JP2 Serotype b Strains of Ghanaian and Swedish Origin.
Front Cell Infect Microbiol
January 2022
Division of Oral Microbiology, Department of Odontology, Umeå University, Umeå, Sweden.
Objective And Methods: The Gram-negative bacterium, is associated with periodontitis affecting young individuals. The geographic dissemination of the highly leukotoxic JP2 genotype of serotype b of this species was previously studied by multilocus sequence typing (MLST). Here, we have used MLST to genetically characterize non-JP2 genotype strains of serotype b, isolated from individuals living in Ghana (n=41), and in Sweden (n=13), respectively.
View Article and Find Full Text PDFDifferential Cell Lysis Among Periodontal Strains of JP2 and Non-JP2 Genotype of Serotype B Is Not Reflected in Dissimilar Expression and Production of Leukotoxin.
Pathogens
October 2019
Section for Pediatric Dentistry, Department of Dentistry and Oral Health, Health, Aarhus University, 8000 Aarhus, Denmark.
Leukotoxic potential of strains has been studied by the use of several methods, and results differ depending on the methods used. The aim of the present study was to perform a comprehensive examination of the leukotoxic potential of a collection of strains by use of three quantitative methods, Western blotting, ELISA, and mRNA expression assay and compare these results with previous data obtained by a cell lysis assay. A higher leukotoxic potential among JP2 genotype strains compared to non-JP2 genotype strains of was found by Western blotting, ELISA and mRNA expression assay.
View Article and Find Full Text PDFGenetic Profiling of Serotype B Isolated from Periodontitis Patients Living in Sweden.
Pathogens
September 2019
Division of Oral Microbiology, Department of Odontology, Umeå University, 907 00 Umeå, Sweden.
The bacterium is associated with aggressive forms of periodontitis and with systemic diseases, such as endocarditis. By assessing a Ghanaian longitudinal adolescent cohort, we earlier recognized the gene as a possible diagnostic marker for a subgroup of JP2 and non-JP2 genotype serotype b strains, associated with high leukotoxicity as determined in a semi-quantitative cell assay. This group of is associated with the progression of attachment loss.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!