AI Article Synopsis
- The tomato mutant is important for hybrid seed breeding as it produces no pollen and has exposed stigmas.
- Histological analysis found issues with pollen and tapetum development, pinpointing a specific gene locus linked to the mutation.
- The study developed a genetic marker for this mutation which could help improve breeding techniques and advance research on pollen development in tomatoes.
Article Abstract
The tomato () () mutant has been used in hybrid seed breeding programs largely because it produces no pollen and has exserted stigmas. In this study, histological examination of anthers revealed dysfunctional pollen and tapetum development in the mutant. The locus was fine mapped to a 28.5 kb interval that encoded four putative genes. , a homolog of Arabidopsis and rice , was proposed to be the candidate gene of because it contained a single nucleotide polymorphism (SNP) that led to the formation of a premature stop codon. A codominant derived cleaved amplified polymorphic sequence (dCAPS) marker, MS32D, was developed based on the SNP. Real-time quantitative reverse-transcription PCR showed that most of the genes, which were proposed to be involved in pollen and tapetum development in tomato, were downregulated in the mutant. These findings may aid in marker-assisted selection of in hybrid breeding programs and facilitate studies on the regulatory mechanisms of pollen and tapetum development in tomato.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6804878 | PMC |
| http://dx.doi.org/10.1038/s41438-019-0170-2 | DOI Listing |
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