AI Article Synopsis
- The research focused on improving tuberculosis vaccines, particularly a recombinant BCG vaccine called rBCG-LTAK63, which showed better protection in mice than the standard BCG vaccine.
- The study analyzed the immune responses generated by both vaccines, finding that rBCG-LTAK63 led to heightened levels of important immune cells like neutrophils and lymphocytes, along with increased production of key signaling molecules.
- Results indicated that rBCG-LTAK63 not only boosted short-term immune responses but also promoted a more robust long-term response, suggesting it could offer superior protection against tuberculosis compared to traditional BCG.*
Article Abstract
The development of more effective vaccines against has become a world priority. Previously, we have shown that a recombinant BCG expressing the LTAK63 adjuvant (rBCG-LTAK63) displayed higher protection than BCG against tuberculosis challenge in mice. In order to elucidate the immune effector mechanisms induced by rBCG-LTAK63, we evaluated the immune response before and after challenge. The potential to induce an innate immune response was investigated by intraperitoneal immunization with BCG or rBCG-LTAK63: both displayed increased cellular infiltration in the peritoneum with high numbers of neutrophils at 24 h and macrophages at 7 d. The rBCG-LTAK63-immunized mice displayed increased production of Nitric Oxide at 24 h and Hydrogen Peroxide at 7 d. The number of lymphocytes was higher in the rBCG-LTAK63 group when compared to BCG. Immunophenotyping of lymphocytes showed that rBCG-LTAK63 immunization increased CD4 and CD8 T cells. An increased long-term Th1/Th17 cytokine profile was observed 90 d after subcutaneous immunization with rBCG-LTAK63. The evaluation of immune responses at 15 d after challenge showed that rBCG-LTAK63-immunized mice displayed increased TNF-α-secreting CD4 T cells and multifunctional IL-2 TNF-α CD4 T cells as compared to BCG-immunized mice. Our results suggest that immunization with rBCG-LTAK63 induces enhanced innate and long-term immune responses as compared to BCG. These results can be correlated with the superior protection induced against TB.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7227645 | PMC |
| http://dx.doi.org/10.1080/21645515.2019.1669414 | DOI Listing |
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