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Root-knot nematode is an important soil pest in horticulture crops and constrains the protected cultivation development after methyl bromide (MB) was phased out in China. Dimethyl disulfide (DMDS) exhibits excellent efficacy against nematodes. Laboratory experiments and field trials were set up to clarify DMDS dose, efficacy, and yield. A dose-response experiment using three methods showed that DMDS presented high efficacy against the nematode Meloidogyne incongnita. The LC50 values of direct fumigation activity in the dessicator method were 0.086 and 0.070 mg L-1 for DMDS and 1,3-D, 29.865 and 18.851 mg L-1 for DMDS and 1,3-D of direct contact activity in the small tube method, 6.438 and 3.061 mg L-1 for DMDS and 1,3-D of soil fumigation activity in the soil fumigation method, respectively. The field trials indicated that DMDS showed an excellent efficacy of 80%-94% on root-knot nematode applied at 10-100 g m-2 on tomato in Tongzhou, Beijing. The crop yields showed no significant difference after applying 10-80 g m-2 DMDS. Results indicate that DMDS applied at 10 g m-2 for controlling root-knot nematode in Beijing is cost effective. In conclusion, DMDS is an excellent soil fumigant that can be used for controlling root-knot nematode and can be an potential novel alternative to MB in China.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6816568 | PMC |
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0224456 | PLOS |
Mol Biol Rep
December 2024
Department of Plant Biotechnology, Centre for Plant Molecular Biology and Biotechnology, (CPMB&B), Tamil Nadu Agricultural University, Coimbatore, 641 037, India.
Background: Tomato (Solanum lycopersicum L.) is a widely cultivated crop in tropical regions, but its production is often hampered by significant losses attributed to diseases like tomato leaf curl virus (ToLCV), fusarium wilt and root-knot nematode.
Methods And Results: This study employed an integrated approach utilizing both co-dominant and dominant SCAR markers, selected for specific resistance genes (ToLCV-Ty-1, Ty-2, Ty-2, Fusarium wilt (Race-2)-I-2, and Root-knot nematode-Mi-1.
Int J Mol Sci
November 2024
Bari Unit, Institute for Sustainable Plant Protection, Department of Biology, Agricultural and Food Sciences, National Research Council of Italy, 70126 Bari, Italy.
The immune response in plants is regulated by several phytohormones and involves the overexpression of defense genes, including the pathogenesis-related () genes. The data reported in this paper indicate that nematodes can suppress the immune response by inhibiting the expression of defense genes. Transcripts from nine defense genes were detected by qRT-PCR in the roots of tomato plants at three and seven days post-inoculation (dpi) with living juveniles (J2s) of (root-knot nematodes, RKNs).
View Article and Find Full Text PDFInt J Mol Sci
November 2024
Facultad de Ciencias Agropecuarias, Universidad de Caldas, Calle 65 No. 26-10, Manizales 170003, Caldas, Colombia.
Meloidogyne causes a devastating disease known as root-knot that affects tomatoes and other cash crops worldwide. Conversely, has proven beneficial in mitigating the effects of various pathogens in plants. We aimed to unravel the molecular events that underlie the beneficial effects of the bacterium and the detrimental impacts of the nematode when inoculated separately or together in tomato plants.
View Article and Find Full Text PDFJ Basic Microbiol
December 2024
Department of Pharmaceutics, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia.
Research on nematode management globally highlights the use of nematicidal biomolecules and biocontrol agents. However, the availability of biomolecules to manage plant-parasitic nematodes remains limited. The discovery of microbial biomolecules offers new opportunities in this field, though they are underexplored for suppressing nematodes.
View Article and Find Full Text PDFPlant Physiol Biochem
November 2024
Beijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops, College of Horticulture, China Agricultural University, Beijing, 100193, China. Electronic address:
Root knot nematodes (RKNs) induce hypertrophy and cell proliferation within the vascular cylinders of host plants, leading to the formation of giant cells (GCs) that are enlarged, multinucleate cells with high metabolic activity. These GCs are formed through repeated karyokinesis without cytokinesis and are accompanied by significant changes in cytoskeleton organization. In this study, two microtubule-binding protein genes, CsMAP65-2 and CsMAP65-3, are upregulated in cucumber roots upon RKNs infection, specifically at 3, 96, and 120 hpi.
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