The objective of this study was to evaluate the effect of the simplified ethanol wet-bonding technique on luting of posts to intraradicular dentin. The effect was assessed by push-out bond strength testing and confocal laser scanning microscopic analysis. Thirty endodontically treated roots were distributed into 3 groups (n = 10): water wet-bonding; stepwise ethanol wet-bonding; and simplified ethanol wet-bonding. After cementation of the posts, the roots were sectioned. Specimens were stored for 24 hours or 1 year before they were stressed to failure. Specimens from each group were processed for microscopic analysis. Data were analyzed using a 2-way analysis of variance and the Tukey test (α = 0.05). Statistically significant differences in push-out bond strength values were observed between the technique groups (P < 0.001) and between the storage periods (P = 0.009). The bond strength results in the group bonded using the simplified ethanol wet-dehydration approach did not differ from those in the group bonded using the stepwise ethanol technique at either storage time interval (P > 0.05). From 24 hours to 1 year, there was a similar decrease in bond strength for specimens prepared with the simplified and stepwise techniques. The microscopic results confirmed that ethanol wet-bonding technique improved the quality of the interface. The simplified ethanol wet-dehydration protocol achieved bond strengths and stability over time that were similar to those obtained with the stepwise ethanol technique and may be considered an alternative strategy to accomplish resin cement-root dentin bonding.
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Molecules
December 2024
Chemical Engineering Department, University of Guadalajara-CUCEI, Blvd. Marcelino García Barragán #1421, esq. Calzada Olímpica, Guadalajara 44430, Jalisco, Mexico.
Tequila vinasses are organic wastes generated during ethanol fermentation at elevated temperatures (≥90 °C) and pH ≤ 4.0, making them hazardous to the environment. This paper describes a new, simplified UV-vis spectroscopy-based procedure for monitoring the adsorption of color compounds in tequila vinasses onto silica-based adsorbents, along with an optimized synthesis method to produce the most efficient sol-gel synthesized thiol-functionalized adsorbent.
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November 2024
Sanitary Engineering Section, Department of Water Management, Delft University of Technology, Delft, Netherlands.
Vinasse, a by-product of ethanol production, is generated at significant rates. While rich in nutrients such as calcium, magnesium, and potassium, its high solids, organic matter, acidity, and sulfate content pose challenges when disposed directly on soil, necessitating treatment. Anaerobic digestion is a viable solution, reducing organic pollution while recovering energy in the form of biogas, aligning with the biorefinery concept.
View Article and Find Full Text PDFInt J Biol Macromol
January 2025
Guangdong Key Laboratory of Fermentation and Enzyme Engineering, School of Biology and Biological Engineering, South China University of Technology, Guangzhou 510006, PR China. Electronic address:
Hydroxytyrosol has been proven beneficial to human health. However, the process involving the conversion of L-DOPA to 3,4-dihydroxyphenylacetaldehyde (3,4-DHPAA) in hydroxytyrosol biosynthesis typically required the simultaneous use of decarboxylase and oxidative deaminase. In addition, phenylacetaldehyde reductase from Solanum lycopersicum (SlPAR) in hydroxytyrosol biosynthesis exhibits poor thermal stability.
View Article and Find Full Text PDFChemosphere
November 2024
Department of Biology, University of Naples Federico II, 80126, Napoli, Italy.
The emission of microfibers (MFs) into all environmental matrices, including biota, is a global concern, but appropriate methodologies aimed at biomonitoring these pollutants are still in an exploratory stage. In this work a new method is presented for the extraction of airborne anthropogenic microfibers (MFs) from the leaves of Pittosporum tobira. The method is a sequential extraction consisting of tape tearing followed by water floating, ethanol extraction, and wet peroxidation, which considered at first outermost MFs, weakly adhering to leaf surface, and deeper MFs partly embedded in waxy cuticle layer.
View Article and Find Full Text PDFEnviron Pollut
December 2024
Department of Environmental Engineering, Seoul National University of Science and Technology, Seoul, 01811, Republic of Korea. Electronic address:
Indoor fungal contamination poses significant challenges to human health and indoor air quality. This study addresses an effective approach using mass spectrometry and machine learning to identify microbial volatile organic compounds (MVOCs) originated from indoor fungi. Three common indoor fungi, including Penicillium Chrysogenum, Cladosporium cladosporioides, and Aspergillus niger, were cultivated on various substrates, namely potato dextrose agar, wallpaper, and silicone.
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