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High-throughput screening strategies for plastic-depolymerizing enzymes.
Trends Biotechnol
January 2025
Key Laboratory of Engineering Biology for Low-Carbon Manufacturing, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China; National Technology Innovation Center of Synthetic Biology, Tianjin 300308, China. Electronic address:
A multitude of plastic-depolymerizing microorganisms and enzymes have been discovered in the plastisphere. Identifying and engineering such microbial strains and enzymes necessitate robust and high-throughput screening strategies for developing effective microbial solutions to counter the plastic accumulation problem and decouple the reliance on fossil resources. This review covers new methods and approaches for the effective high-throughput screening of depolymerizing enzymes for various plastics, such as polyethylene terephthalate (PET), polyurethane (PU), and polylactic acid (PLA).
View Article and Find Full Text PDFIntrinsic dual-emitting Si dots for high-precision and broad-range pH detection.
Anal Chim Acta
February 2025
Shenzhen Key Laboratory of Synthetic Genomics, Guangdong Provincial Key Laboratory of Synthetic Genomics, CAS Key Laboratory of Quantitative Engineering Biology, Shenzhen Institute of Synthetic Biology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518055, China. Electronic address:
Background: High-precision and broad-range pH detection is critical for health status assessment, such as signal transduction, enzyme activity, endocytosis, and cell proliferation and apoptosis. Although pH-responsive ratiometric fluorescent probes offer an effective pH monitoring strategy, their preparation often requires multi-step modification and decreases fluorescence efficiency and stability. Herein, we developed a simple method to prepare fluorescent Si dots with dual emission centers for high-precision and broad-range pH monitoring, and the detection of urease based on pH-responsive Si dots and pH monitoring in living cell was further explored.
View Article and Find Full Text PDFIn situ surface oxygen vacancy effect synergistic with internal polarization effect in BiFeO for photoelectrochemical detection of T4 DNA ligase.
Talanta
January 2025
Key Laboratory of Synthetic and Biological Colloids (Ministry of Education), School of Chemical and Material Engineering, Jiangnan University, Wuxi, 214122, China. Electronic address:
Recently, the field of cathode photoelectrochemistry has advanced significantly, yet there remained a dearth of innovative approaches in signal transmission strategies. This paper introduced a novel concept where the dopamine (DA)-engineered surface vacancy (Ov) effect on BiFeO microspheres synergistically interacted with the intrinsic polarization of the material, leading to a significantly enhanced photocurrent when compared to that of BiO or FeO alone without a built-in electric field. Based on this finding, we proposed a PEC biosensor that leveraged the competitive binding reaction between single-base nucleotides and DA for photocurrent output, wherein the T4 DNA ligase-mediated ligation reaction governed the production of single-base nucleotides.
View Article and Find Full Text PDFEngineering of L-threonine and L-proline biosensors by directed evolution of transcriptional regulator SerR and application for high-throughput screening.
Bioresour Bioprocess
January 2025
Key Laboratory of Engineering Biology for Low-carbon Manufacturing, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, China.
Amino acids are important bio-based products with a multi-billion-dollar market. The development of efficient high-throughput screening technologies utilizing biosensors is essential for the rapid identification of high-performance amino acid producers. However, there remains a pressing need for biosensors that specifically target certain critical amino acids, such as L-threonine and L-proline.
View Article and Find Full Text PDFA dual-mode biosensor for microRNA detection based on DNA tetrahedron-gated nanochannels.
Mikrochim Acta
January 2025
Key Laboratory of Synthetic and Natural Functional Molecule, College of Chemistry and Materials Science, Northwest University, Xi'an, 710127, People's Republic of China.
A biosensor based on solid-state nanochannels of anodic aluminum oxide (AAO) membrane for both electrochemical and naked-eye detection of microRNA-31 (MiR-31) is proposed. For this purpose, MoS nanosheets, which possess different adsorption capabilities to single-stranded and double-stranded nucleic acids, are deposited onto the top surface of the AAO membrane. Moreover, multi-functional DNA nanostructure have been designed by linking a G-rich sequence for folding to a G-quadruplex at three vertices and a complementary sequence of MiR-31 at the other one vertex of a DNA tetrahedron.
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