Modulation of Glucosinolate Composition in Brassicaceae Seeds by Germination and Fungal Elicitation.

J Agric Food Chem

Laboratory of Food Chemistry , Wageningen University, P.O. Box 17, 6700 AA Wageningen , The Netherlands.

Published: November 2019

Glucosinolates (GSLs) are of interest for potential antimicrobial activity of their degradation products and exclusive presence in Brassicaceae. Compositional changes of aliphatic, benzenic, and indolic GSLs of , , and seeds by germination and fungal elicitation were studied. (nonpathogenic), (nonpathogenic), and (pathogenic) were employed. Thirty-one GSLs were detected by reversed-phase ultrahigh-performance liquid chromatography photodiode array with in-line electrospray ionization mass spectrometry (RP-UHPLC-PDA-ESI-MS). Aromatic-acylated derivatives of 3-butenyl GSL, -hydroxybenzyl GSL, and indol-3-ylmethyl GSL were for the first time tentatively annotated and confirmed to be not artifacts. For , germination, elicitation, and elicitation increased total GSL content, mainly consisting of -hydroxybenzyl GSL, by 2-3 fold. For and , total GSL content was unaffected by germination or elicitation. In all treatments, aliphatic GSL content was decreased (≥50%) in and remained unchanged in . Indolic GSLs were induced in all species by germination and nonpathogenic elicitation.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6873265PMC
http://dx.doi.org/10.1021/acs.jafc.9b05771DOI Listing

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